{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T06:15:26Z","timestamp":1776838526119,"version":"3.51.2"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,4,4]],"date-time":"2024-04-04T00:00:00Z","timestamp":1712188800000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Single\u2010cell RNA sequencing analysis has been usually conducted on post\u2010traumatic epilepsy (PET) and hereditary epilepsy (HE) patients; however, the transcriptome of patients with traumatic temporal lobe epilepsy has rarely been studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Hippocampus tissues isolated from one patient with PTE and one patient with HE were used in the present study. Single cell isolates were prepared and captured using a 10\u00d7 Genomics Chromium Single\u2010Cell 3\u2032 kit (V3) according to the manufacturer's instructions. The libraries were sequenced on an Illumina NovaSeq 6000 sequencing system. Raw data were processed, and the cells were filtered and classified using the Seurat R package. Uniform Manifold Approximation and Projection was used for visualization. Differentially expressed genes (DEGs) were identified based on a <jats:italic>p<\/jats:italic>\u2010value \u22640.01 and log fold change (FC) \u22650.25. Gene Ontology (GO, <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/geneontology.org\/\">http:\/\/geneontology.org\/<\/jats:ext-link>) and KEGG (Kyoto Encyclopedia of Genes and Genomes, <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.genome.jp\/kegg\">www.genome.jp\/kegg<\/jats:ext-link>) analyses were performed on the DEGs for enrichment analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The reads obtained from the 10\u00d7 genomic platform for PTE and HE were 39.56\u2009M and 30.08\u2009M, respectively. The Q30 score of the RNA reads was &gt;91.6%. After filtering, 7479 PTE cells and 9357 HE cells remained for further study. More than 96.4% of the reads were mapped to GRCh38\/GRCm38. The cells were differentially distributed in two groups, with higher numbers of oligodendrocytes (6522 vs. 2532) and astrocytes (133 vs. 52), and lower numbers of microglial cells (2242 vs. 3811), and neurons (3 vs. 203) present in the HE group than in the PTE group. The DEGs in four cell clusters were identified, with 25 being in oligodendrocytes (13 upregulated and 12 downregulated), 87 in microglia cells (42 upregulated and 45 downregulated), 222 in astrocytes (115 upregulated and 107 downregulated), and 393 in neurons (305 upregulated and 88 downregulated). The genes <jats:italic>MTND1P23<\/jats:italic> (downregulated), <jats:italic>XIST<\/jats:italic> (downregulated), and <jats:italic>RPS4Y1<\/jats:italic> (upregulated) were commonly expressed in all four cell clusters. The DEGs in microglial cells and astrocytes were enriched in the IL\u201017 signaling pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our study explored differences in cells found in a patient with PE compared to a patient with HE, and the transcriptome in the different cells was analyzed for the first time. Studying inflammatory and immune functions might be the best approach for investigating traumatic temporal lobe epilepsy in neurons.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.14702","type":"journal-article","created":{"date-parts":[[2024,4,4]],"date-time":"2024-04-04T11:31:38Z","timestamp":1712230298000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Molecular mechanism analyses of post\u2010traumatic epilepsy and hereditary epilepsy based on 10\u00d7 single\u2010cell transcriptome sequencing technology"],"prefix":"10.1111","volume":"30","author":[{"given":"Fang","family":"Wen","sequence":"first","affiliation":[{"name":"Department of Neurology The Second Xiang\u2010Ya Hospital of Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhigang","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery The Second Xiang\u2010Ya Hospital of Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dezhi","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery The Second Xiang\u2010Ya Hospital of Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4854-670X","authenticated-orcid":false,"given":"Jun","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery The Second Xiang\u2010Ya Hospital of Central South University  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,4]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)32596-0"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(21)00023-5"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2018.06.022"},{"issue":"4","key":"e_1_2_10_5_1","article-title":"Post\u2010traumatic seizures: a deep\u2010dive into pathogenesis","volume":"13","author":"Anwer F","year":"2021","journal-title":"Cureus"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000012744"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3109\/02699052.2015.1005131"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2020.106825"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/epi.16794"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yebeh.2019.106594"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1008120"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2021.01.026"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aav8130"},{"key":"e_1_2_10_14_1","doi-asserted-by":"crossref","unstructured":"Tome\u2010GarciaJ NudelmanG MussaZ et\u00a0al.Cell type\u2010specific isolation and transcriptomic profiling informs glial pathology in human temporal lobe epilepsy. 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