{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T20:28:08Z","timestamp":1774988888191,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T00:00:00Z","timestamp":1719878400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82171301"],"award-info":[{"award-number":["82171301"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82371306"],"award-info":[{"award-number":["82371306"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005089","name":"Beijing Municipal Natural Science Foundation","doi-asserted-by":"publisher","award":["7222083"],"award-info":[{"award-number":["7222083"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Ischemic stroke remains a challenge in medical research because of the limited treatment options. Recombinant human tissue plasminogen activator (rtPA) is the primary treatment for recanalization. However, nearly 50% of the patients experience complications that result in ineffective reperfusion. The precise factors contributing to ineffective reperfusion remain unclear; however, recent studies have suggested that immune cells, notably neutrophils, may influence the outcome of rtPA thrombolysis via mechanisms such as the formation of neutrophil extracellular traps. This study aimed to explore the nonthrombolytic effects of rtPA on neutrophils and highlight their contribution to ineffective reperfusion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We evaluated the effects of rtPA treatment on middle cerebral artery occlusion in rats. We also assessed neutrophil infiltration and activation after rtPA treatment in\u00a0vitro and in\u00a0vivo in a small cohort of patients with massive cerebral ischemia (MCI).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>rtPA increased neutrophil infiltration into the brain microvessels and worsened blood\u2013brain barrier damage during ischemia. It also increased the neutrophil counts of the patients with MCI.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Neutrophils play a crucial role in promoting ischemic injury and blood\u2013brain barrier disruption, making them potential therapeutic targets.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.14825","type":"journal-article","created":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T19:05:36Z","timestamp":1719947136000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Neutrophil migration participates in the side effect of recombinant human tissue plasminogen activator"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5830-1207","authenticated-orcid":false,"given":"Yuyou","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Neurology and Institute of Cerebrovascular Diseases Research Xuanwu Hospital of Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziping","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Neurology and Institute of Cerebrovascular Diseases Research Xuanwu Hospital of Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tong","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Neurology and Institute of Cerebrovascular Diseases Research Xuanwu Hospital of Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yangmin","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Neurology and Institute of Cerebrovascular Diseases Research Xuanwu Hospital of Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenhong","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Neurology and Institute of Cerebrovascular Diseases Research Xuanwu Hospital of Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junfen","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Neurology and Institute of Cerebrovascular Diseases Research Xuanwu Hospital of Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rongliang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology and Institute of Cerebrovascular Diseases Research Xuanwu Hospital of Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Neurology and Institute of Cerebrovascular Diseases Research Xuanwu Hospital of Capital Medical University  Beijing 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