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Cystometrogram (CMG), simultaneous CMG, and external urethral sphincter electromyography (EUS\u2010EMG) were conducted in all groups under urethane anesthesia. Drugs were administered intrathecally during CMG and EUS\u2010EMG. Rats were euthanized and L6\u2010S1 spinal cord were acquired for immunofluorescence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In SCI rats, intrathecal administration of 2,5\u2010dimethoxy\u20104\u2010iodophenyl\u20102\u2010aminopropane hydrochloride or L\u2010type VGCC blocker, nifedipine, could significantly increase voiding volume, voiding efficiency, and the number of high\u2010frequency oscillations. They could also prolong EUS bursting activity duration on EUS\u2010EMG. Moreover, the effect of 2,5\u2010dimethoxy\u20104\u2010iodophenyl\u20102\u2010aminopropane hydrochloride can be eliminated with the combined administration of L\u2010type VGCC agonist, (\u00b1)\u2010Bay K 8644. No significant differences were observed in CMG after intrathecal administration of T\u2010type VGCC blocker TTA\u2010P2. Additionally, immunofluorescence of the lumbosacral cord in control and SCI rats showed that the 5\u2010HT<jats:sub>2A<\/jats:sub> receptor and Cav1.2 immunolabeling\u2010positive neurons in the anterior horn of the lumbosacral cord were increased in SCI rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our study demonstrated that 5\u2010HT<jats:sub>2A\/2C<\/jats:sub> agonist 2,5\u2010dimethoxy\u20104\u2010iodophenyl\u20102\u2010aminopropane hydrochloride may improve SCI\u2010induced DSD by inhibiting the L\u2010type voltage\u2010gated calcium channel in lumbosacral cord motoneurons.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.14890","type":"journal-article","created":{"date-parts":[[2024,8,4]],"date-time":"2024-08-04T16:45:40Z","timestamp":1722789940000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Serotonin (5\u2010<scp>HT<\/scp>)<sub><scp>2A<\/scp>\/<scp>2C<\/scp><\/sub> receptor agonist 2,5\u2010dimethoxy\u20104\u2010iodophenyl\u20102\u2010aminopropane hydrochloride improves detrusor sphincter dyssynergia by inhibiting L\u2010type voltage\u2010gated calcium channels in spinal cord injured rats"],"prefix":"10.1111","volume":"30","author":[{"given":"Rong","family":"Lv","sequence":"first","affiliation":[{"name":"Department of Urology Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingzhuo","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Urology Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Urology Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengtian","family":"Li","sequence":"additional","affiliation":[{"name":"Bio\u2010X Institutes, Key laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Key Laboratory of Psychotic Disorders, Brain Science and Technology Research Center Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nailong","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Urology Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5090-3767","authenticated-orcid":false,"given":"Baojun","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Urology Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao 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