{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T22:07:10Z","timestamp":1780524430905,"version":"3.54.1"},"reference-count":48,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T00:00:00Z","timestamp":1727136000000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Resting state functional magnetic resonance imaging (rs\u2010fMRI) has been widely used in studying default mode network (DMN) changes in postoperative delirium (POD). Reproducibility and interpretability of the analyzing results remain insufficiently studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Delirium\u2010like behavior was induced by tibial fixation surgery under isoflurane anesthesia. Firstly, we evaluated delirium\u2010like behavior and inflammatory responses in hippocampus and systemic level. Then the expressions of microRNA (miRNA) and target gene were sequenced and validated. Afterwards the functional connectivity (FC) in DMN was analyzed. Finally, results were correlated with DMN changes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>POD\u2010like behavior caused significant changes of miR\u201034b\u20105p, miR\u2010328\u20105p, and miR\u20103505 in miRNA level and <jats:italic>Nos1<\/jats:italic>, <jats:italic>Tubb3<\/jats:italic>, and <jats:italic>Gys1<\/jats:italic> in the gene level. The FC in left and right hippocampus (L\u2010Hip and R\u2010Hip) and right auditory cortex (R\u2010AC) was found significantly changed. Significant correlations were found in FC<jats:sub>L\u2010Hip\/R\u2010AC<\/jats:sub> and FC<jats:sub>R\u2010Hip\/R\u2010AC<\/jats:sub> for miR\u201034b\u20105p and miR\u20103505, as well as <jats:italic>Nos1<\/jats:italic> and <jats:italic>Tubb3<\/jats:italic>. For miR\u2010328\u20105p, no significant correlations were found.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our study demonstrates that POD\u2010like behavior induced significant miRNA and gene expression changes were associated with hippocampus related long\u2010term FC disruption in DMN. The results increased reproducibility and interpretability for standardized rs\u2010fMRI data analysis, as well as providing potential targets for postoperative delirium treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.70038","type":"journal-article","created":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T02:43:14Z","timestamp":1727232194000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Expression of microRNA induced by postoperative delirium\u2010like behavior is associated with long\u2010term default mode network disruption: Sequencing and a secondary analysis of resting\u2010state fMRI data"],"prefix":"10.1111","volume":"30","author":[{"given":"Yang","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Xuanwu Hospital, Capital Medical University  Beijing China"},{"name":"National Clinical Research Center for Geriatric Diseases  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiru","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Xuanwu Hospital, Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiqun","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Xuanwu Hospital, Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binbin","family":"Nie","sequence":"additional","affiliation":[{"name":"Institute of High Energy Physics Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6291-2505","authenticated-orcid":false,"given":"Tianlong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Xuanwu Hospital, Capital Medical University  Beijing China"},{"name":"National Clinical Research Center for Geriatric Diseases  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12871-019-0903-7"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bja.2020.06.063"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2020.8931"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jclinane.2021.110496"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2022.1068278"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2021.118503"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2023.04.023"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-neuro-071013-014030"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.13387"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awab218"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2023.1076824"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-018-0235-4"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2020.117461"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2766-y"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.13794"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-021-02330-z"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12640-022-00597-1"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2022.960657"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/ans.14258"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2022.1013478"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-018-0207-0"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-023-02845-3"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2013.155"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-8994-2_9"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2014.08.002"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13041-023-00998-z"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12877-023-03923-0"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00221-020-06007-2"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/ACO.0000000000000536"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mri.2017.07.011"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/hbm.21511"},{"key":"e_1_2_10_33_1","first-page":"386","article-title":"GRETNA: a graph theoretical network analysis toolbox for imaging connectomics","volume":"9","author":"Wang J","year":"2015","journal-title":"Front Hum Neurosci"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41398-021-01304-y"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1177\/0004867419848826"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-020-00812-1"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11481-019-09857-y"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2023.105479"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.15436"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.14902"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bja.2022.07.054"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/npp.2016.40"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.13433"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2020.116877"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bja.2017.11.087"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaneurol.2020.2273"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-2010-101491"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2022.102326"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0910834107"}],"container-title":["CNS Neuroscience &amp; Therapeutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cns.70038","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T03:57:41Z","timestamp":1727755061000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cns.70038"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9]]},"references-count":48,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1111\/cns.70038"],"URL":"https:\/\/doi.org\/10.1111\/cns.70038","archive":["Portico"],"relation":{},"ISSN":["1755-5930","1755-5949"],"issn-type":[{"value":"1755-5930","type":"print"},{"value":"1755-5949","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9]]},"assertion":[{"value":"2024-07-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70038"}}