{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T15:22:47Z","timestamp":1789226567177,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T00:00:00Z","timestamp":1739750400000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2025,2]]},"abstract":"ABSTRACT<\/jats:title>\n \n Objective<\/jats:title>\n This study aimed to investigate the impact of ischemic stroke (IS) on sleep homeostasis and circadian rhythms in rats, as well as the underlying mechanisms.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n The middle cerebral artery occlusion model was employed to induce IS in rats. Sixty young and sixty aged rats were randomly divided into six groups for experiments. Neurological function was assessed using the Garcia score, and infarct size was evaluated through 2,3,5\u2010triphenyltetrazolium chloride staining. Sleep\u2013wake cycles were monitored by implanting electrodes into the neck muscles to record electroencephalograms and electromyograms. Parameters such as sleep latency, waking time, non\u2010rapid eye movement (NREM) sleeping, rapid eye movement sleeping, NREM delta power, and waking theta power were measured. Serum cortisol and melatonin levels were measured using enzyme\u2010linked immunosorbent assay. Gene and protein expression of circadian regulators period 1 (\n Per1<\/jats:italic>\n ) and cryptochrome 1 (\n Cry1<\/jats:italic>\n ) in the pineal gland were assessed using real\u2010time quantitative reverse transcription polymerase chain reaction and western blot.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Compared to the sham groups, IS\u2010induced rats showed a decrease in Garcia scores and an increase in cerebral infarction area. Besides, relative to young rats, aged rats exhibited more severe cerebral infraction damage, lower melatonin levels, higher cortisol levels, disrupted sleep\u2013wake cycles, and altered gene and protein expression levels of\n Per1<\/jats:italic>\n and\n Cry1<\/jats:italic>\n in the pineal gland.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n IS can lead to neurological impairments and brain damage, with aged rats showing more severe effects. IS also disturbs melatonin and cortisol levels, affects sleep homeostasis, and results in disordered\n Per1<\/jats:italic>\n and\n Cry1<\/jats:italic>\n gene and protein expression levels. These findings underscore the role of circadian disruption and stress response in the pathology of IS, especially in aging populations.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/cns.70153","type":"journal-article","created":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T03:54:21Z","timestamp":1739764461000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Study on the Mechanisms of Ischemic Stroke Impacting Sleep Homeostasis and Circadian Rhythms in Rats"],"prefix":"10.1111","volume":"31","author":[{"given":"Ting\u2010ting","family":"Chu","sequence":"first","affiliation":[{"name":"Department of Neurology Fourth Affiliated Hospital of Harbin Medical University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of General Surgery The 2nd Affiliated Hospital of Harbin Medical University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u2010hui","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Neurology Fourth Affiliated Hospital of Harbin Medical University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010ying","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Neurology Fourth Affiliated Hospital of Harbin Medical University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin\u2010lin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Neurology Fourth Affiliated Hospital of Harbin Medical University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6647-6748","authenticated-orcid":false,"given":"Jing\u2010yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Neurology Fourth Affiliated Hospital of Harbin Medical University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,17]]},"reference":[{"issue":"8","key":"e_1_2_13_2_1","article-title":"Correction to: An Updated Definition of Stroke for the 21st Century: A Statement for Healthcare Professionals From the American Heart Association\/American Stroke Association","volume":"50","year":"2019","journal-title":"Stroke"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(21)00252-0"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11325-016-1454-5"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000006904"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12395"},{"key":"e_1_2_13_7_1","volume-title":"Biological Effects of Cortisol","author":"Kamgang V. 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