{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T21:45:03Z","timestamp":1771537503851,"version":"3.50.1"},"reference-count":68,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,20]],"date-time":"2025-01-20T00:00:00Z","timestamp":1737331200000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002799","name":"Capital Medical University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002799","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Ischemia\u2013reperfusion of the abdominal aorta often results in damage to distant organs, such as the heart and brain. This cellular heterogeneity within affected tissues complicates the roles of specific cell subsets in abdominal aorta occlusion model (AAO) injury. However, cell type\u2013specific molecular pathology in the hippocampus after ischemia is poorly understood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aims<\/jats:title><jats:p>In this study, we adopted a mouse AAO to investigate the single\u2010cell transcriptome in the hippocampi in AAO mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Male C57BL\/6 mice (8\u2009weeks old) were used to create an AAO model, with animals divided into Sham and I\/R groups. The I\/R group was subjected to 2\u2009h of ischemia followed by 24\u2009h of reperfusion, after which hippocampal tissues were collected for single\u2010cell RNA sequencing and histological analysis. Behavioral tests, including the Rotarod, Y\u2010maze, and new object recognition tests, were performed daily for 28\u2009days post\u2010surgery to evaluate neurological function. A total of 62,624 cells were corresponding 7 cell types with neuronal, glial, and vascular lineages. We next analyzed cell\u2010specific gene alterations in AAO mice and the function of these cell\u2010specific Genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>AAO injury upregulated astrocyte and oligodendrocyte precursor cell (OPC) proportions (<jats:italic>p<\/jats:italic>\u2010value &lt;\u20090.05). Astrocytes showed unique gene expression related to neurogenesis and mRNA processing. Five distinct astrocyte subtypes emerged post\u2010injury. OPCs exhibited enhanced synapse organization. Microglia activation and the elevated expression level of the epithelial cell oxidative phosphorylation protein\u2013protein interaction (PPI) module indicate an inflammatory response and metabolic changes in response to AAO injury.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our scRNA\u2010seq analysis provides insights into transcriptional changes at the single\u2010cell level in response to AAO\u2010induced hippocampal injury. This study illustrates how the hippocampal region responds to such injury and identifies potential therapeutic targets for intervention, thereby paving the way for future research and treatment strategies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.70154","type":"journal-article","created":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T07:17:25Z","timestamp":1737443845000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Single\u2010Cell Insights Into Cellular Response in Abdominal Aortic Occlusion\u2010Induced Hippocampal Injury"],"prefix":"10.1111","volume":"31","author":[{"given":"Changhong","family":"Ren","sequence":"first","affiliation":[{"name":"Beijing Key Laboratory of Hypoxia Translational Medicine, Xuanwu Hospital, Center of Stroke, Beijing Institute of Brain Disorder Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1416-1601","authenticated-orcid":false,"given":"Ling","family":"Kui","sequence":"additional","affiliation":[{"name":"Bioinformatics Center Shenzhen Qianhai Shekou Free Trade Zone Hospital  Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Xu","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Hypoxia Translational Medicine, Xuanwu Hospital, Center of Stroke, Beijing Institute of Brain Disorder Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Tong","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Hypoxia Translational Medicine, Xuanwu Hospital, Center of Stroke, Beijing Institute of Brain Disorder Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojie","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology Shenzhen Qianhai Shekou Free Trade Zone Hospital  Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianping","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Neurology Shenzhen Qianhai Shekou Free Trade Zone Hospital  Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaomei","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Interventional Radiology, Senior Department of Oncology Fifth Medical Center of PLA General Hospital  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoyun","family":"Wang","sequence":"additional","affiliation":[{"name":"Bioinformatics Center Shenzhen Qianhai Shekou Free Trade Zone Hospital  Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng\u2010Yong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Interventional Radiology, Senior Department of Oncology Fifth Medical Center of PLA General Hospital  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sijie","family":"Li","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Hypoxia Translational Medicine, Xuanwu Hospital, Center of Stroke, Beijing Institute of Brain Disorder Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0527-2852","authenticated-orcid":false,"given":"Xunming","family":"Ji","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Hypoxia Translational Medicine, Xuanwu Hospital, Center of Stroke, Beijing Institute of Brain Disorder Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,20]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.5606\/tgkdc.dergisi.2021.21737"},{"issue":"1","key":"e_1_2_12_3_1","first-page":"77","article-title":"Thymoquinone Protects End Organs From Abdominal Aorta Ischemia\/Reperfusion Injury in a Rat Model","volume":"30","author":"Aydin M. 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