{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T03:49:55Z","timestamp":1777952995596,"version":"3.51.4"},"reference-count":54,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T00:00:00Z","timestamp":1739750400000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002383","name":"King Saud University","doi-asserted-by":"publisher","award":["RSPD2025R744"],"award-info":[{"award-number":["RSPD2025R744"]}],"id":[{"id":"10.13039\/501100002383","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Numerous studies suggest that morellic acid (MOR), highly available in Garcinia plants, has different physiological activities, including anti\u2010cancer, anti\u2010oxidant, and anti\u2010microbial activity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>\n                      In this investigation, we aimed to demonstrate the anxiolytic activity, along with the mechanism behind this activity of MOR, using in\u00a0vivo and\n                      <jats:italic>in silico<\/jats:italic>\n                      studies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      For this, we used different doses of MOR (5 and 10\u2009mg\/kg) and administered this drug intraperitoneally to\n                      <jats:italic>Swiss<\/jats:italic>\n                      albino mice (male and female). Diazepam (DZP), a positive allosteric modulator of the GABA\n                      <jats:sub>A<\/jats:sub>\n                      receptor, was used as a positive control at a dose of 2\u2009mg\/kg (i.p), and vehicle was used as a control group. In this test, various test protocols are used to assess the behavioral patterns of mice, including swing, hole cross, light\u2013dark testing, and open field testing.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      This investigation revealed that MOR remarkably reduced the locomotor activity of mice in a dose\u2010dependent manner and produced calming behaviors like DZP. However, the findings showed that the combination of MOR and DZP synergistically reduced the locomotion of mice compared to the single therapy. On the other hand, from the computational study, the result demonstrated that MOR exhibited the highest binding scores (\u22129.2\u2009kcal\/mol) towards the GABA\n                      <jats:sub>A<\/jats:sub>\n                      receptor \u03b13 subunit and \u22127.6\u2009kcal\/mol towards the GABA\n                      <jats:sub>A<\/jats:sub>\n                      \u03b12 receptor. Whereas, DZP showed \u22126.6 and \u22127.3\u2009kcal\/mol docking affinity and FLU exerted \u22126.2 and \u22126.3\u2009kcal\/mol docking scores towards the GABA\n                      <jats:sub>A<\/jats:sub>\n                      receptor \u03b12 and \u03b13 subunits, respectively. The ligand interacted with the receptor by forming different hydrogen and hydrophobic bonds.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>However, it is recommended that more precise and comprehensive preclinical investigations be required to demonstrate the exact mechanism behind the anxiolytic effects and conduct clinical trials to determine efficacy and safety.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cns.70276","type":"journal-article","created":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T05:18:41Z","timestamp":1739769521000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Anxiolytic Activity of Morellic Acid: Modulation of Diazepam's Anxiolytic Effects, Possibly Through\n                    <scp>GABAergic<\/scp>\n                    Interventions"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6179-9720","authenticated-orcid":false,"given":"Md. Shimul","family":"Bhuia","sequence":"first","affiliation":[{"name":"Department of Pharmacy Bangabandhu Sheikh Mujibur Rahman Science and Technology University  Dhaka Bangladesh"},{"name":"Bioinformatics and Drug Innovation Laboratory BioLuster Research Center Ltd.  Dhaka Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanzila Akter","family":"Eity","sequence":"additional","affiliation":[{"name":"Bioinformatics and Drug Innovation Laboratory BioLuster Research Center Ltd.  Dhaka Bangladesh"},{"name":"Department of Biotechnology and Genetic Engineering Bangabandhu Sheikh Mujibur Rahman Science and Technology University  Dhaka Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0672-6581","authenticated-orcid":false,"given":"Raihan","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Bangabandhu Sheikh Mujibur Rahman Science and Technology University  Dhaka Bangladesh"},{"name":"Bioinformatics and Drug Innovation Laboratory BioLuster Research Center Ltd.  Dhaka Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siddique Akber","family":"Ansari","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, College of Pharmacy King Saud University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehedi Hasan","family":"Bappi","sequence":"additional","affiliation":[{"name":"Bioinformatics and Drug Innovation Laboratory BioLuster Research Center Ltd.  Dhaka Bangladesh"},{"name":"School of Pharmacy Jeonbuk National University  Jeonju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md. Anin","family":"Nayeem","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Bangabandhu Sheikh Mujibur Rahman Science and Technology University  Dhaka Bangladesh"},{"name":"Bioinformatics and Drug Innovation Laboratory BioLuster Research Center Ltd.  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Dhaka Bangladesh"},{"name":"Pharmacy Discipline Khulna University  Khulna Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,2,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/archpsyc.62.6.617"},{"key":"e_1_2_9_3_1","first-page":"227","article-title":"The Development of Anxiety: The Role of Control in the Early Environment","volume":"2","author":"Chorpita B. F.","year":"2018","journal-title":"Psychological Bulletin"},{"issue":"4","key":"e_1_2_9_4_1","article-title":"Association between anxiety and depression in Medical students at Universidad Ricardo Palma during the year 2021","volume":"22","author":"Nina M. 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