{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T03:49:47Z","timestamp":1777952987301,"version":"3.51.4"},"reference-count":57,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T00:00:00Z","timestamp":1740441600000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Piwi\u2010interacting RNAs (piRNAs) are differentially expressed after cerebral ischemia. However, little is known about their roles in transient global cerebral ischemia (tGCI). Herein, we aim to elucidate the roles and the underlying molecular mechanisms of piRNAs in tGCI and cerebral ischemic tolerance induced by hypoxic postconditioning (HPC).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The male rat models of tGCI and HPC were established in\u00a0vivo. Oxygen\u2013glucose deprivation\/reoxygenation (OGD\/R) was developed from primary hippocampal neurons in\u00a0vitro. RNA\u2010sequencing, fluorescence in\u00a0situ hybridization, and quantitative real\u2010time PCR were used for detecting piRNA expression. Immunohistochemistry, TUNEL staining, CCK8 assay, etc., were used to evaluate neuronal damage. Western blot was used to measure protein levels of NR2B, PSD95, and cleaved\u2010caspase 3.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The expression profiles of piRNAs in CA1 were significantly changed after tGCI. HPC downregulated the expression of the top 5 piRNAs associated with synaptic function. Notably, the knockdown of rno_piR_011022 not only alleviated neuronal apoptosis and enhanced synaptic plasticity after tGCI and OGD\/R but also reduced methyl\u2010D\u2010aspartate (NMDA) receptor 2B (NR2B) expression and inhibited NR2B\u2010postsynaptic density 95 (PSD95) interaction following tGCI. HPC enhanced these inhibitory effects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This innovative study indicated that the down\u2010regulation of rno_piR_011022 plays an important role in HPC\u2010mediated neuroprotection against tGCI through inhibiting the NR2B\u2010PSD95 interaction.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cns.70295","type":"journal-article","created":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T22:35:34Z","timestamp":1740522934000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Hypoxic Postconditioning Offers Neuroprotection Against Transient Cerebral Ischemia via Down\u2010Regulation of rno_piR_011022"],"prefix":"10.1111","volume":"31","author":[{"given":"Meiyan","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Neurology, Institute of Neuroscience, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, the Second Affiliated Hospital Guangzhou Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanshan","family":"Duan","sequence":"additional","affiliation":[{"name":"Department of Neurology, Institute of Neuroscience, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, the Second Affiliated Hospital Guangzhou Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guorong","family":"Chai","sequence":"additional","affiliation":[{"name":"Department of Neurology, Institute of Neuroscience, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, the Second Affiliated Hospital Guangzhou Medical University  Guangzhou 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