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Following mRNA sequencing of all astrocyte groups, astrocyte apoptosis and reactivity were analyzed through a combined intervention of rrTSG\u20106 and Apoptozole, a heat shock protein 70\u20101B (Hsp70\u20101B) inhibitor, in\u00a0vitro.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The findings demonstrated that rrTSG\u20106 significantly reduced cerebral infarct volumes by nearly half, improved neurobehavioral outcomes, mitigated BBB damage, and suppressed the expressions of astrocyte apoptosis markers, reactivity indicators, and A1 phenotype markers. mRNA sequencing revealed that the Hsp70\u20101B protein level increased to approximately 1.6 times that of the rrTSG\u20106 non\u2010intervention group. Furthermore, Apoptozole impeded the expressions of astrocyte apoptosis markers, reactivity indicators, and A1 phenotype markers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>TSG\u20106 inhibited nuclear factor kappa\u2010B (NF\u2010\u03baB) phosphorylation by upregulating Hsp70\u20101B in oxygen\u2013glucose deprivation\/reoxygenation (OGD\/R)\u2010induced astrocytes, thereby exerting a protective effect through anti\u2010apoptotic mechanisms and the suppression of astrocyte reactivity and A1 transformation following cerebral I\/R injury.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.70354","type":"journal-article","created":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T05:20:49Z","timestamp":1743052849000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>TSG<\/scp>\u20106 Protects Against Cerebral Ischemia\u2013Reperfusion Injury via Upregulating Hsp70\u2010<scp>1B<\/scp> in 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