{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T14:41:26Z","timestamp":1754145686372,"version":"3.41.2"},"reference-count":63,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T00:00:00Z","timestamp":1748908800000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870521","82270820"],"award-info":[{"award-number":["81870521","82270820"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>This study aimed to investigate brain activity using functional magnetic resonance imaging (fMRI) in rats with 12\u2010week diabetic bladder dysfunction (DBD). Furthermore, as prior research has suggested that NLX\u2010112, a 5\u2010HT<jats:sub>1A<\/jats:sub> receptor agonist, may alleviate DBD, we sought to explore its effects on brain activity in DBD rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Male Sprague\u2013Dawley rats were anesthetized with urethane and underwent cystometry alongside 9.4\u2010Tesla fMRI evaluations. Resting\u2010state fMRI was performed on empty bladders to compare baseline brain activity between groups. Gradient echo\u2010planar imaging was utilized to assess brain activation during micturition relative to relaxation, and a group analysis was conducted.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>fMRI data from 12 diabetes mellitus (DM) rats and 12 normal control (NC) rats were analyzed. DM rats exhibited a significant reduction in the amplitude of low\u2010frequency fluctuation (ALFF) in the basal forebrain and cerebral cortex, a measurement of activity in brain networks. During micturition, DM rats showed increased activation in the thalamus, primary motor cortex, periaqueductal gray (PAG) and other regions. NLX\u2010112 administration did not significantly alter brain activity in DM rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>DBD rats exhibit heightened thalamic and PAG activity during micturition, potentially due to enlarged bladder capacity, with cortical activity serving as a compensatory mechanism. These findings highlight potential neural targets for DBD treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.70466","type":"journal-article","created":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T05:33:14Z","timestamp":1748928794000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Functional <scp>MRI<\/scp> Analysis of Brain Activity in Rats With Diabetic Bladder Dysfunction"],"prefix":"10.1111","volume":"31","author":[{"given":"Mingzhuo","family":"Li","sequence":"first","affiliation":[{"name":"Department of Urology Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Urology Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai 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