{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:49:36Z","timestamp":1790830176492,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T00:00:00Z","timestamp":1751500800000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2406703"],"award-info":[{"award-number":["2022YFC2406703"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>To improve the timeliness and accuracy of anti\u2010epilepsy drug monitoring, we developed a point\u2010of\u2010care mass spectrometry (PoC MS) for real\u2010time quantification of valproic acid (VPA), as a paradigm for optimizing perioperative management.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The PoC MS integrates a miniature mass spectrometer, paper capillary spray, and selective ion isolation. A total of 119 blood samples were analyzed for VPA quantification. An equivalent calibration model was established with 12 paired serum and whole blood samples. Analytical performance was benchmarked against liquid chromatography\u2010mass spectrometry (LC\u2013MS) and enzyme multiplied immunoassay technique (EMIT) with 50 whole blood samples. A validation cohort of 45 samples collected at different time points from 9 patients was analyzed to monitor perioperative VPA concentration dynamics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Selective ion isolation could improve signal intensity by threefold and enable VPA quantification down to 10\u2009\u03bcg\/mL within 3\u2009min, demonstrating a clear speed advantage over other methods. The calibration model showed excellent agreement between whole blood and serum concentrations (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>\u2009=\u20090.978). PoC MS measurements of VPA closely matched LC\u2013MS (<jats:italic>r<\/jats:italic>\u2009=\u20090.990, bias\u2009=\u20090.16%) and EMIT (<jats:italic>r<\/jats:italic>\u2009=\u20090.988, bias\u2009=\u20095.4%). In clinic, VPA level varied by up to 15\u2010fold across patients, and bedside PoC MS could depict dynamic VPA concentration profiles, with intra\u2010patient fluctuations averaging 26%, thereby supporting drug monitoring and therapeutic decision\u2010making.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>PoC MS could enable rapid, accurate, and matrix\u2010tolerant bedside monitoring of VPA from whole blood, supporting personalized antiseizure therapy and real\u2010time clinical decision.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.70499","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T04:39:38Z","timestamp":1751517578000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Real\u2010Time Monitoring of the Antiseizure Drug Valproic Acid Using a Novel Point\u2010Of\u2010Care Mass Spectrometry"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2391-6196","authenticated-orcid":false,"given":"Xinqi","family":"Fang","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, Huashan Hospital, Shanghai Medical College Fudan University  Shanghai China"},{"name":"National Center for Neurological Disorders  Shanghai China"},{"name":"Shanghai Key Laboratory of Brain Function and Restoration and Neural Regeneration  Shanghai China"},{"name":"Neurosurgical Institute of Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junhan","family":"Wu","sequence":"additional","affiliation":[{"name":"PURSPEC Technology (China) Ltd.  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