{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T05:35:10Z","timestamp":1791956110181,"version":"4.3.4"},"reference-count":52,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T00:00:00Z","timestamp":1753228800000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82260452"],"award-info":[{"award-number":["82260452"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["gyfynsfc [2023] 16"],"award-info":[{"award-number":["gyfynsfc [2023] 16"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>To explore the impact of intermittent theta burst stimulation (iTBS) treatment at various targets on spinal cord injury (SCI), as well as the effects of dual\u2010target iTBS therapy on neurological functional recovery in rats with SCI and its underlying mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using an improved Allen's method, an incomplete C6 SCI model was established. Postoperatively, the rats with SCI underwent transcranial iTBS, trans\u2010spinal iTBS, or dual\u2010target iTBS. Neurological functional recovery and synaptic function following SCI were evaluated through behavioral tests, footprint analysis, electrophysiological assessments, pathological staining, transmission electron microscopy, Golgi staining, and immunofluorescence staining. The expression of relevant proteins and genes was assessed using Western blotting and qRT\u2010PCR. Proteomic and metabolomic sequencing analyses of spinal cord tissue from each group were conducted to investigate specific mechanisms. Additionally, lentivirus was used to infect primary neurons to elucidate the effect of PDE1A. Furthermore, lentivirus was applied to SCI rats to explore the influence of PDE1A on neurological and synaptic functions following SCI.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with the single\u2010target iTBS group, dual\u2010target iTBS treatment significantly improved motor function and reduced the damaged area of the spinal cord in SCI rats. Following dual\u2010target iTBS intervention, SCI rats exhibited improvements in neural function and synaptic function. Sequencing analysis identified the protein PDE1A present in all groups, and the protein interaction network revealed that PDE1A is involved in the cAMP signaling pathway, with an increase in PDE1A expression observed after SCI. Additionally, inhibiting PDE1A promoted the expression of cAMP and protein kinase cAMP\u2010activated catalytic subunit alpha (PRKACA) in primary neurons, thereby facilitating synapse function in primary neurons. Inhibition of PDE1A also improved neural connection and synaptic reconstruction in SCI rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Compared with single\u2010target iTBS treatment, dual\u2010target iTBS treatment promotes the recovery of motor function and spinal cord tissue repair more effectively in SCI rats. Dual\u2010target iTBS may promote neural regeneration and synaptic remodeling after SCI by regulating the PDE1A\u2010cAMP\u2010PKA signaling pathway, thereby improving neurological dysfunction.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.70525","type":"journal-article","created":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T05:34:46Z","timestamp":1753335286000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Transcranial i<scp>TBS<\/scp> Combined With Trans\u2010Spinal i<scp>TBS<\/scp> Targeting <scp>PDE<\/scp>1<scp>A<\/scp>\/c<scp>AMP<\/scp>\/<scp>PKA<\/scp> Axis Regulates Neural Regeneration After Spinal Cord Injury"],"prefix":"10.1111","volume":"31","author":[{"given":"Yingxue","family":"Fu","sequence":"first","affiliation":[{"name":"Department of Rehabilitation Medicine The Affiliated Hospital of Guizhou Medical University  Guiyang Guizhou China"},{"name":"Department of Clinical Medicine Guizhou Medical University  Guian New District Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianbin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medicine The Affiliated Hospital of Guizhou Medical University  Guiyang Guizhou China"},{"name":"Department of Clinical Medicine Guizhou Medical University  Guian New District Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingyu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medicine The Affiliated Hospital of Guizhou Medical University  Guiyang Guizhou China"},{"name":"Department of Clinical Medicine Guizhou Medical University  Guian New District Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8925-4610","authenticated-orcid":false,"given":"Shuang","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medicine The Affiliated Hospital of Guizhou Medical University  Guiyang Guizhou China"},{"name":"Department of Clinical Medicine Guizhou Medical University  Guian New District Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.317961"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.wneu.2018.02.033"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-022-00562-y"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/procel\/pwad003"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2023.1099143"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2017.09.005"},{"key":"e_1_2_8_8_1","article-title":"Intrinsic Plasticity Mechanisms of Repetitive Transcranial Magnetic Stimulation","volume":"30","author":"King E. 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