{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T13:42:43Z","timestamp":1791726163581,"version":"4.3.4"},"reference-count":53,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,27]],"date-time":"2025-07-27T00:00:00Z","timestamp":1753574400000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82071257"],"award-info":[{"award-number":["82071257"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82371254"],"award-info":[{"award-number":["82371254"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Parkinson's disease (PD) patients experience gut microbiota dysbiosis. Probiotic intervention could potentially serve as a safe and effective adjunctive therapeutic approach for PD, but its effects on rapid eye movement sleep behavior disorder (RBD) and motor symptoms in PD patients warrant further investigation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To examine the influence of probiotics on RBD, motor symptoms, gut microbiota, and serum metabolites in individuals with PD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this randomized controlled trial, PD patients were randomly allocated to either a probiotics or a control group while maintaining standard treatments. Clinical outcomes, including Unified Parkinson's Disease Rating Scale (UPDRS) and RBD Questionnaire\u2010Hong Kong (RBDQ\u2010HK) were assessed at baseline and post\u2010treatment. Furthermore, fecal and blood samples were collected from PD patients at both timepoints, with additional samples obtained from healthy controls for comparison. The 16S rRNA gene V3\u2010V4 region sequencing method was used to analyze gut microbiota composition, and untargeted metabolomic techniques were utilized to assess serum metabolite alterations, followed by correlation analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fifty eligible PD patients were enrolled and randomly allocated into two groups. After 12\u2009weeks of intervention, the probiotic group showed significant reductions in both UPDRS total scores (\u22124.8\u2009\u00b1\u20097.5 vs. 1.8\u2009\u00b1\u200913.1, <jats:italic>p<\/jats:italic>\u2009=\u20090.009) and RBDQ\u2010HK scores (\u22127.5\u2009\u00b1\u20096.5 vs. 0\u2009\u00b1\u20095.8, <jats:italic>p<\/jats:italic>\u2009=\u20090.015) compared to controls. Gut microbiota analysis revealed increased abundance of <jats:italic>Actinobacteria<\/jats:italic>, <jats:italic>Negativicutes<\/jats:italic>, and <jats:italic>Bacillus<\/jats:italic>, with reductions in <jats:italic>Lactococcus<\/jats:italic>, <jats:italic>Comamonas<\/jats:italic>, and <jats:italic>Enterococcus<\/jats:italic> after probiotic intervention. Furthermore, compared to normal controls, PD patients exhibited 9 significantly elevated and 11 significantly reduced metabolites; probiotic intervention altered the serum metabolome in PD patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study demonstrated probiotics' potential to ameliorate RBD and motor symptoms while positively affecting the composition of the gut microbiota and serum metabolites in PD patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.70541","type":"journal-article","created":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T06:54:33Z","timestamp":1753685673000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Effect of Probiotics Supplementation on <scp>REM<\/scp> Sleep Behavior Disorder and Motor Symptoms in Parkinson's Disease: A Pilot Study"],"prefix":"10.1111","volume":"31","author":[{"given":"Yitong","family":"Du","sequence":"first","affiliation":[{"name":"Department of Neurology Beijing Friendship Hospital, Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology Beijing Friendship Hospital, Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Neurology Beijing Friendship Hospital, Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojiao","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Neurology The First Medical Centre, Chinese PLA General Hospital  Beijing 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Capital Medical University, Beijing Shijingshan Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiya","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology Beijing Friendship Hospital, Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Houzhen","family":"Tuo","sequence":"additional","affiliation":[{"name":"Department of Neurology Beijing Friendship Hospital, Capital Medical University  Beijing 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