{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T03:25:30Z","timestamp":1790220330078,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T00:00:00Z","timestamp":1754352000000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871300"],"award-info":[{"award-number":["81871300"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018542","name":"Natural Science Foundation of Sichuan Province","doi-asserted-by":"publisher","award":["2025NSFSC2253"],"award-info":[{"award-number":["2025NSFSC2253"]}],"id":[{"id":"10.13039\/501100018542","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Multiple Sclerosis (MS) is a neuroinflammatory and neurodegenerative disease affecting the central nervous system (CNS). Substantial evidence implicates a central role for CD4+ T cells in MS pathogenesis, particularly IFN\u2010\u03b3+ Th1 cells and IL\u201017+ Th17 cells. NF\u2010\u03baB plays an essential role in regulating the differentiation of Th1 and Th17 cells, which typically mediate inflammatory responses as self\u2010triggers. QNZ is a highly selective inhibitor of NF\u2010\u03baB transcriptional activation. In this study, we assessed the impact of QNZ on CD4+ T\u2010cell polarization in MS. Utilizing the experimental autoimmune encephalomyelitis (EAE) model, we investigated these aspects of MS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>EAE was induced in C57BL\/6 female mice by active immunization with myelin oligodendrocyte glycoprotein (MOG)<jats:sub>35\u201355<\/jats:sub> peptide. QNZ was injected intraperitoneally (<jats:italic>i.p<\/jats:italic>.) once every 2\u2009days after the first immunization. Disease severity was clinically assessed and histopathologically assessed in the CNS. Phenotyping of CD4+ T cells was performed by flow cytometry in the spleen and cervical lymph nodes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Prophylactic administration of QNZ to EAE mice suppressed the differentiation of Th1 and Th17 cells and demyelination within the spinal cord. Notably, QNZ also reduced the proportion of IFN\u2010\u03b3+IL\u201017+ Th17.1 cells, potentially playing a critical role in MS pathogenesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Quinazoline derivative QNZ could suppress neuroinflammation, alleviate the progression of EAE and be associated with reduced Th1 and Th17 immunity.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.70555","type":"journal-article","created":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T04:49:18Z","timestamp":1754455758000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The Quinazoline Derivative, <scp>QNZ<\/scp>, Alleviates Experimental Autoimmune Encephalomyelitis by Suppressing Th1 and Th17 Cells"],"prefix":"10.1111","volume":"31","author":[{"given":"Fan","family":"Yang","sequence":"first","affiliation":[{"name":"Clinical Laboratory Clinical Medical College and The First Affiliated Hospital of Chengdu 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