{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T04:20:04Z","timestamp":1789014004674,"version":"build-2803163510"},"reference-count":55,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T00:00:00Z","timestamp":1755475200000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["92148206"],"award-info":[{"award-number":["92148206"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100031312","name":"Justis- og beredskapsdepartementet","doi-asserted-by":"publisher","award":["2023BAA005"],"award-info":[{"award-number":["2023BAA005"]}],"id":[{"id":"10.13039\/100031312","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Transient ischemic attack (TIA) serves as a crucial precursor to a potential stroke. Angiogenesis is essential for the renovation of the damaged brain after TIA. This study aimed to elucidate the role of miRNA\u201021a\u20105p in angiogenesis following TIA and unravel the underlying mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In vivo and in\u00a0vitro TIA models were established using the modified suture middle cerebral artery occlusion and oxygen\u2013glucose deprivation\/reoxygenation methods. Differentially expressed miRNAs in the TIA group versus the control group were identified using small RNA sequencing. The putative target genes of miRNA\u201021a\u20105p were predicted via bioinformatics analysis and validated using a dual luciferase reporter gene assay. TIA models were then treated with miRNA\u201021a\u20105p antagomir\/agomir or\/and an adeno\u2010associated virus interfering with the target gene to assess the effects of miRNA\u201021a\u20105p and the identified target gene on angiogenesis after TIA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MiRNA\u201021a\u20105p was the miRNA with the most significant changes after TIA. RBMS3 was identified as a target gene for miRNA\u201021a\u20105p. Downregulation of miRNA\u201021a\u20105p expression reduced angiogenesis in animal and cellular models of TIA, and miRNA\u201021a\u20105p upregulation had a completely opposite effect. RBMS3 suppression reversed miRNA\u201021a\u20105p knockdown\u2010mediated inhibition of angiogenesis in TIA models. Moreover, the TGFBR1\/SMAD2\/3 pathway was found to be downstream for miRNA<jats:bold>\u2010<\/jats:bold>21a\u20105p\/RBMS3.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>MiRNA\u201021a\u20105p conferred neuroprotective effects against TIA by enhancing angiogenesis through the RBMS3\/TGFBR1\/SMAD2\/3 pathway.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cns.70573","type":"journal-article","created":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T07:06:52Z","timestamp":1755500812000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>MicroRNA<\/scp>\u201021a\u20105p Promotes Cerebral Angiogenesis in Transient Ischemic Attack by Targeting <scp>RBMS3<\/scp> and Subsequently Modulating the <scp>TGFBR1<\/scp>\/<scp>SMAD2<\/scp>\/3 Pathway"],"prefix":"10.1111","volume":"31","author":[{"given":"Jiahui","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Neurology, Tongji 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