{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T12:04:38Z","timestamp":1792065878450,"version":"4.4.0"},"reference-count":48,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T00:00:00Z","timestamp":1756339200000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100017962","name":"Jiangsu Commission of Health","doi-asserted-by":"publisher","award":["LKM2023034"],"award-info":[{"award-number":["LKM2023034"]}],"id":[{"id":"10.13039\/100017962","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Compulsive relapse (reinstatement) behavior of methamphetamine underlies the difficulty of withdrawal and is associated with abnormal BDNF\u2010mediated synaptic plasticity. However, how to intervene in this aberrant synaptic plasticity to prevent its reinstatement behavior in mice has not fully been studied.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The CPP was used to establish a model of methamphetamine\u2010induced reinstatement behavior in C57BL\/6 mice. Intraperitoneal injections of TEN were administered during the remission phase after the successful establishment of the CPP model to investigate the therapeutic effects on reinstatement. Immunofluorescence experiments were used to detect c\u2010fos expression in hippocampal CA1 neurons. Electrophysiological methods were used to determine glutamatergic transmission in hippocampal CA1 neural circuits. Western blotting was used to detect BDNF\/TrKB and PSD\u201095 protein expressions. Molecular docking was used to predict TEN molecule\u2013protein binding.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Compared with control mice, METH\u2010treated mice presented increased CPP scores during the reinstatement phase, whereas, compared to METH\u2010treated mice, TEN\u2010treated mice presented significantly lower CPP scores. Immunofluorescence experiments indicated that TEN was able to inhibit the METH\u2010induced increase in c\u2010fos content. In addition, we found that TEN alleviates the METH\u2010triggered increase in glutamatergic transmission in mouse hippocampal CA1 neurons. Importantly, molecular docking studies demonstrated that TEN binds with BDNF, which may be important targets for its biological function. We also demonstrated that interfering with BDNF inhibits the therapeutic effect of TEN on the reinstatement of METH addiction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings suggest that TEN treats METH\u2010induced reinstatement behavior by binding to BDNF, which may provide a novel target for treating relapse in patients addicted to METH.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cns.70588","type":"journal-article","created":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T07:09:51Z","timestamp":1756364991000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Tenuifolin Attenuates Methamphetamine\u2010Induced Reinstatement in Mice by Regulating Hippocampal Postsynaptic\n                    <scp>BDNF<\/scp>\n                    Signaling"],"prefix":"10.1111","volume":"31","author":[{"given":"Yize","family":"Qi","sequence":"first","affiliation":[{"name":"Institute for Stem Cell and Neural Regeneration and Key Laboratory of Cardiovascular &amp;Cerebrovascular Medicine, School of Pharmacy Nanjing Medical University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyuan","family":"Fan","sequence":"additional","affiliation":[{"name":"Institute for Stem Cell and Neural Regeneration and Key Laboratory of Cardiovascular &amp;Cerebrovascular Medicine, School of Pharmacy Nanjing Medical University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Substance Dependence The Affiliated Xuzhou Eastern Hospital of Xuzhou Medical University, Xuzhou Eastern People's Hospital  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanqing","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Substance Dependence The Affiliated Xuzhou Eastern Hospital of Xuzhou Medical University, Xuzhou Eastern People's Hospital  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Substance Dependence The Affiliated Xuzhou Eastern Hospital of Xuzhou Medical University, Xuzhou Eastern People's Hospital  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3259-6942","authenticated-orcid":false,"given":"Qingfeng","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Substance Dependence The Affiliated Xuzhou Eastern Hospital of Xuzhou Medical University, Xuzhou Eastern People's Hospital  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,28]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.278.5335.45"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn3105"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.15212\/npt-2024-0003"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamapsychiatry.2020.0246"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.392887"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2106648118"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-7254.2006.00431.x"},{"issue":"3","key":"e_1_2_10_9_1","first-page":"431","article-title":"Current Situation and Progress of Drug Abuse in Chinese Mainland","volume":"42","author":"Qi Y.","year":"2022","journal-title":"Journal of Nanjing Medical University. 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