{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T14:19:23Z","timestamp":1791555563514,"version":"4.3.4"},"reference-count":46,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T00:00:00Z","timestamp":1762473600000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82130034"],"award-info":[{"award-number":["82130034"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82221001"],"award-info":[{"award-number":["82221001"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81971035"],"award-info":[{"award-number":["81971035"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>\n                      While acute itch comprises histamine\u2010dependent and \u2010independent subtypes, critical mechanisms underlying histamine\u2010independent itch remain poorly understood. This study investigates the role of paired\u2010like homeobox\u00a02a (Phox2a) in tachykinin 1\u2010positive (Tac1\n                      <jats:sup>+<\/jats:sup>\n                      ) neurons of the lateral spinal nucleus (LSN) as a novel target for histamine\u2010independent pruritus intervention.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We combined chemogenetic manipulation (viral\u2010mediated neuronal activation\/inhibition), whole\u2010cell patch\u2010clamp recordings, immunohistochemistry, fluorescence in\u00a0situ hybridization, Western blotting, and behavioral assays to investigate the role of LSN\n                      <jats:sup>Tac1<\/jats:sup>\n                      neurons and Phox2a in itch modulation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      LSN\n                      <jats:sup>Tac1<\/jats:sup>\n                      neurons were specifically activated during chloroquine (CQ)\u2013induced histamine\u2010independent itch. Chemogenetic activation of these neurons exacerbated scratching, whereas inhibition suppressed itch behavior. Notably, Phox2a, expressed in LSN\n                      <jats:sup>Tac1<\/jats:sup>\n                      neurons, was downregulated during CQ\u2010induced itch. Overexpression of Phox2a in LSN\n                      <jats:sup>Tac1<\/jats:sup>\n                      neurons significantly alleviated CQ\u2010evoked scratching and was accompanied by a reduction in spontaneous excitatory postsynaptic currents (sEPSCs) amplitude without a change in sEPSCs frequency.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Our findings identify Phox2a in LSN\n                      <jats:sup>Tac1<\/jats:sup>\n                      neurons as a selective regulator of histamine\u2010independent acute itch through presynaptic excitability. This highlights Phox2a as a novel therapeutic target for histamine\u2010independent pruritus intervention.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cns.70639","type":"journal-article","created":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T05:26:43Z","timestamp":1762579603000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Phox2a in Lateral Spinal Nucleus\n                    <i>Tac1<\/i>\n                    \u2010Positive Neurons Mediates Histamine\u2010Independent Acute Itch"],"prefix":"10.1111","volume":"31","author":[{"given":"Yu\u2010Ling","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Human Anatomy and Histology and Embryology, School of Basic Medical Sciences The Fourth Military Medical University  Xi'an China"},{"name":"Department of Human Anatomy Baotou Medical College  Baotou 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