{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T18:20:26Z","timestamp":1775845226182,"version":"3.50.1"},"reference-count":61,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,3,13]],"date-time":"2015-03-13T00:00:00Z","timestamp":1426204800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["Grant-in-Aid for Scientific Research (C) no. 25462656"],"award-info":[{"award-number":["Grant-in-Aid for Scientific Research (C) no. 25462656"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Otolaryngology"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>We investigated the electrical impedance of and the expressions of tight junction molecules in the cholesteatoma epithelium to provide supporting evidence for the acid lysis theory of bone resorption in middle ear cholesteatoma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Study subjects were patients with primary acquired middle ear cholesteatoma and those with non\u2010cholesteatomatous chronic otitis media who underwent tympanomastoidectomy. The electrical impedance of the cholesteatoma epithelium was measured during tympanomastoidectomy by loading alternating currents of 320\u00a0Hz and 30.7\u00a0kHz. The expressions of <jats:italic>tricellulin<\/jats:italic> (<jats:italic>MARVELD2<\/jats:italic>), <jats:italic>claudin\u20101<\/jats:italic> (<jats:italic>CLDN1<\/jats:italic>) and <jats:italic>claudin\u20103<\/jats:italic> (<jats:italic>CLDN3<\/jats:italic>) were examined by fluorescence immunohistochemistry and quantitative reverse transcription\u2010polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The electrical impedance of the cholesteatoma epithelium was significantly lower than that of the post\u2010auricular skin and external auditory canal skin at both 320\u00a0Hz and 30.7\u00a0kHz. Immunoreactivity for MARVELD2, CLDN1 and CLDN3 was localised mainly in the granular layer, and to lesser degree, in the horny and spinous layers in both the cholesteatoma tissue and post\u2010auricular skin. Fluorescence intensity was moderate for MARVELD2, weak for CLDN1 and strong for CLDN3. The expressions of <jats:italic>MARVELD2<\/jats:italic>,<jats:italic> CLDN1<\/jats:italic> and <jats:italic>CLDN3<\/jats:italic> m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> were significantly lower in the cholesteatoma tissue than in the post\u2010auricular skin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results indicate the increased permeability of the cholesteatoma epithelium and suggest that this change is, at least partially, dependent on the decrease in the expressions of the tight junction molecules. This evidence supports the acid lysis hypothesis of bone resorption in cholesteatoma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/coa.12332","type":"journal-article","created":{"date-parts":[[2014,10,16]],"date-time":"2014-10-16T06:53:43Z","timestamp":1413442423000},"page":"106-114","source":"Crossref","is-referenced-by-count":19,"title":["Increased permeability of the epithelium of middle ear cholesteatoma"],"prefix":"10.1111","volume":"40","author":[{"given":"H.","family":"Koizumi","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery University of Occupational and Environmental Health Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery University of Occupational and Environmental Health Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Ohbuchi","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery University of Occupational and Environmental Health Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Kitamura","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery University of Occupational and Environmental Health Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Hashida","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery University of Occupational and Environmental Health Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Dermatology School of Medicine University of Occupational and Environmental Health Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,3,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.5540870513"},{"key":"e_1_2_9_3_1","first-page":"197","article-title":"Prostaglandins in human cholesteatoma and granulation tissue","volume":"9","author":"Jung T.T.","year":"1988","journal-title":"Am. 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