{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T17:01:16Z","timestamp":1746637276719,"version":"3.37.3"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,7,15]],"date-time":"2015-07-15T00:00:00Z","timestamp":1436918400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Otolaryngology"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Fascin is an actin\u2010bundling protein found in cell membrane protrusions and increases cell motility. The expression of fascin in epithelial neoplasms has been described only recently. No data are available concerning the role of this protein in invasive cholesteatoma. Thus, we investigated the expression of fascin in cholesteatoma tissue and the relationship between fascin expression and intraoperative evaluation of the destruction of the ossicular chain and extent of disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Cholesteatoma specimens of 28 patients and external auditory canal (<jats:styled-content style=\"fixed-case\">EAC<\/jats:styled-content>) skin specimens of the same patients (as the control group) were collected from mastoidectomies. Immunohistochemical technique was used to investigate the fascin expression in all cholesteatoma tissues and <jats:styled-content style=\"fixed-case\">EAC<\/jats:styled-content> skin specimens. Immunohistochemical staining was assessed semiquantitatively based on the thickness of epithelium. <jats:styled-content style=\"fixed-case\">SPSS<\/jats:styled-content> software version 16.0 (<jats:styled-content style=\"fixed-case\">SPSS<\/jats:styled-content> Inc., Chicago, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> USA<\/jats:styled-content>) was performed to statistically analyse the relationships between fascin expression and intraoperative evaluation destruction of ossicular chain and extent of the disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Immunohistochemically, there was no or very low fascin expression observed in normal epithelial cells of <jats:styled-content style=\"fixed-case\">EAC<\/jats:styled-content> skin, while expressed in cholesteatoma tissue. Also, fascin expression in cholesteatoma tissues was significantly correlated with destruction of ossicular chain and extent of the disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Fascin expression is usually found in cholesteatoma epithelium and is correlated with destruction of the ossicular chain and extent of disease. Considering all of the correlations between the clinical and histopathological findings, \u2018fascin immunoexpression scoring\u2019 may be used for histological grading of cholesteatoma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/coa.12373","type":"journal-article","created":{"date-parts":[[2015,1,9]],"date-time":"2015-01-09T16:24:11Z","timestamp":1420820651000},"page":"335-340","source":"Crossref","is-referenced-by-count":4,"title":["Fascin expression in cholesteatoma: correlation with destruction of the ossicular chain and extent of disease"],"prefix":"10.1111","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2797-0248","authenticated-orcid":false,"given":"A.","family":"Binnetoglu","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery Marmara University Pendik Training and Research Hospital Istanbul 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