{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T21:24:26Z","timestamp":1760909066695},"reference-count":17,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,1,8]],"date-time":"2017-01-08T00:00:00Z","timestamp":1483833600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Otolaryngology"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>The aim of this study was to assess the feasibility of testing actionable mutations in small amounts of formalin\u2010fixed paraffin\u2010embedded material in multiple genes of the receptor tyrosine kinase pathway and to determine the frequency of these mutations in human papillomavirus (<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>)\u2010positive and <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>\u2010negative oropharyngeal cancer (<jats:styled-content style=\"fixed-case\">OPC<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>A retrospective pilot study was performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>In <jats:styled-content style=\"fixed-case\">OPC<\/jats:styled-content>, no predictive markers for response to epidermal growth factor receptor inhibition are known. Therefore, identifying predictive biomarkers is of utmost importance, but is often hampered by the small amount of tumour material available.<\/jats:p><\/jats:sec><jats:sec><jats:title>Participants<\/jats:title><jats:p>We included the archival material of 45 <jats:styled-content style=\"fixed-case\">OPC<\/jats:styled-content>, all treated with concomitant chemoradiotherapy between 2003 and 2010.<\/jats:p><\/jats:sec><jats:sec><jats:title>Main outcome measures<\/jats:title><jats:p>Besides the <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> status, we assessed mutations using a gene panel that targets 16 genes in the receptor tyrosine kinase pathway and six other genes. The polymerase chain reaction required only 10 ng <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In total, 42 of the 45 biopsies have been successfully analysed. In total 20 of 42 samples were <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>\u2010positive and 22 of 42 were <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>\u2010negative. In the receptor tyrosine kinase pathway, mutations in <jats:italic><jats:styled-content style=\"fixed-case\">PIK<\/jats:styled-content>3<jats:styled-content style=\"fixed-case\">CA<\/jats:styled-content><\/jats:italic> were most frequently identified. A <jats:italic><jats:styled-content style=\"fixed-case\">TP<\/jats:styled-content>53<\/jats:italic> mutation was identified in one <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>\u2010positive sample and in 13 <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>\u2010negative samples. Additionally, three mutations in three different genes were found.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We evaluated an assay to identify mutations in the receptor tyrosine kinase pathway. As only small amounts of formalin\u2010fixed paraffin\u2010embedded material are sufficient for reliable analysis, this test opens up new possibilities for personalised medicine.<\/jats:p><\/jats:sec>","DOI":"10.1111\/coa.12800","type":"journal-article","created":{"date-parts":[[2016,11,24]],"date-time":"2016-11-24T07:42:53Z","timestamp":1479973373000},"page":"681-686","source":"Crossref","is-referenced-by-count":1,"title":["Using a semi\u2010conductor sequencing\u2010based panel for genotyping of <scp>HPV<\/scp>\u2010positive and <scp>HPV<\/scp>\u2010negative oropharyngeal cancer: a retrospective pilot study"],"prefix":"10.1111","volume":"42","author":[{"given":"J.C.","family":"Ham","sequence":"first","affiliation":[{"name":"Department of Medical Oncology Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.B.J.","family":"Tops","sequence":"additional","affiliation":[{"name":"Department of Pathology Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.M.L.","family":"Driessen","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.W.M.","family":"van Raaij","sequence":"additional","affiliation":[{"name":"Department of Pathology Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.J.","family":"Slootweg","sequence":"additional","affiliation":[{"name":"Department of Pathology Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.J.G.","family":"Melchers","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.J.L.","family":"Ligtenberg","sequence":"additional","affiliation":[{"name":"Department of Pathology Radboud University Medical Center Nijmegen The Netherlands"},{"name":"Department of Human Genetics Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.M.L.","family":"van Herpen","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,1,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.05.2308"},{"key":"e_1_2_7_3_1","unstructured":"Organization IAfRoCWH.GLOBOCAN(2012)Estimated Cancer Incidence Mortality and Prevalence Worldwide in 2012. 2012. URL:http:\/\/globocan.iarc.fr\/Pages\/fact_sheets_population.aspx"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0912217"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/92.9.709"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdr544"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2011.36.4596"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdt574"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14129"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-13-3310"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijrobp.2008.12.088"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa053422"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0802656"},{"key":"e_1_2_7_14_1","unstructured":"Health USNIo. Available from: Clinicaltrials.gov"},{"key":"e_1_2_7_15_1","first-page":"6469","article-title":"Detection of human papillomavirus in cervical lymph nodes: a highly effective strategy for localizing site of tumor origin","volume":"9","author":"Begum S.","year":"2003","journal-title":"Clin. Cancer Res."},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65462-4"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-015-1015-5"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11404"}],"container-title":["Clinical Otolaryngology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcoa.12800","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/coa.12800","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T18:40:17Z","timestamp":1696358417000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/coa.12800"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1,8]]},"references-count":17,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,6]]}},"alternative-id":["10.1111\/coa.12800"],"URL":"https:\/\/doi.org\/10.1111\/coa.12800","archive":["Portico"],"relation":{},"ISSN":["1749-4478","1749-4486"],"issn-type":[{"value":"1749-4478","type":"print"},{"value":"1749-4486","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,1,8]]}}}