{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T15:24:11Z","timestamp":1773674651799,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,3,18]],"date-time":"2019-03-18T00:00:00Z","timestamp":1552867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16K11203"],"award-info":[{"award-number":["16K11203"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["2016-2018"],"award-info":[{"award-number":["2016-2018"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Otolaryngology"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>We investigated the difference in ciliary beat responsiveness to acetylcholine in ex vivo and the difference in the expressions of associated molecules (M1\/M3 muscarinic receptors, pannexin\u20101 and P2X7 purinergic receptor) between the nasal polyp and turbinate mucosa.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study design<\/jats:title><jats:p>Laboratorial study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Participants<\/jats:title><jats:p>Nasal polyp and inferior turbinate were collected from patients with hypertrophic rhinitis and\/or nasal polyp during endoscopic sinonasal surgery.<\/jats:p><\/jats:sec><jats:sec><jats:title>Main outcome measures<\/jats:title><jats:p>The mucosa was cut into thin strips, and ciliary movement was observed under a phase\u2010contrast light microscope equipped with a high\u2010speed digital video camera. The samples were also examined by scanning electron microscopy, fluorescence immunohistochemistry, and quantitative reverse transcription\u2010polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cilia were well preserved in both tissues at the ultrastructural level. The baseline ciliary beat frequency (CBF) was not different between the two tissues. The CBF of the turbinate was significantly increased by stimulation with acetylcholine (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001), but that of the polyp was not. The ratio of the acetylcholine\u2010stimulated CBF to the baseline CBF was significantly lower in the polyp than in the turbinate (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001). Immunohistochemical study revealed that immunoreactivities for M3, pannexin\u20101 and P2X7 were weaker in the polyp than in the turbinate. The mRNA expressions of M1, M3 and P2X7 were significantly lower and that of pannexin\u20101 tended to be lower in the polyp than in the turbinate.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results indicate that ciliary beat responsiveness to acetylcholine is decreased in the nasal polyp. This may be explained by the decreased expressions of M3, P2X7 and probably pannexin\u20101 in this tissue.<\/jats:p><\/jats:sec>","DOI":"10.1111\/coa.13312","type":"journal-article","created":{"date-parts":[[2019,2,14]],"date-time":"2019-02-14T14:46:24Z","timestamp":1550155584000},"page":"356-365","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Decreased ciliary beat responsiveness to acetylcholine in the nasal polyp epithelium"],"prefix":"10.1111","volume":"44","author":[{"given":"Hung Ba","family":"Do","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery, School of Medicine University of Occupational and Environmental Health Kitakyushu Japan"},{"name":"Department of Otorhinolaryngology Hanoi Medical University Hanoi Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toyoaki","family":"Ohbuchi","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery, School of Medicine University of Occupational and Environmental Health Kitakyushu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuru","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"Shared\u2010Use Research Center, School of Medicine University of Occupational and Environmental Health Kitakyushu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takuro","family":"Kitamura","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery, School of Medicine University of Occupational and Environmental Health Kitakyushu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuro","family":"Wakasugi","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery, School of Medicine University of Occupational and Environmental Health Kitakyushu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun\u2010ichi","family":"Ohkubo","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery, School of Medicine University of Occupational and Environmental Health Kitakyushu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5262-1939","authenticated-orcid":false,"given":"Hideaki","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery, School of Medicine University of Occupational and Environmental Health Kitakyushu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,3,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2500\/ajr.2006.20.2870"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/00016480510037906"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3233\/BIR-1990-273-434"},{"key":"e_1_2_9_5_1","first-page":"17","article-title":"Differentiation of human surface upper airway epithelial cells in primary culture on a floating collagen gel","volume":"2","author":"Chevillard M","year":"1993","journal-title":"Epithelial Cell Biol"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022215100138551"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2002.028704"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.11-202184"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00143.2012"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b12-01076"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/1945892418770292"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.154.6.8970383"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.132.2.179"},{"key":"e_1_2_9_14_1","first-page":"163","article-title":"Impact of inferior turbinate hypertrophy on the aerodynamic pattern and physiological functions of the turbulent airflow \u2010 a CFD simulation model","volume":"48","author":"Chen XB","year":"2010","journal-title":"Rhinology"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.22089"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.132.6.588"},{"key":"e_1_2_9_17_1","first-page":"477","article-title":"Allergic nasal polyps under scanning electron microscope","volume":"7","author":"Skladzen J","year":"2001","journal-title":"Med Sci Monit"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2273.1996.tb01084.x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2014.07.038"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-7158.2011.01409.x"},{"key":"e_1_2_9_21_1","first-page":"136","article-title":"The roles of muscarinic receptor subtypes in modulation of nasal ciliary action","volume":"34","author":"Yang B","year":"1996","journal-title":"Rhinology"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348940211100705"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/03655230701599321"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00228.2008"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11302-009-9146-2"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2009.11.007"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601378"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/phy2.227"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2004.028670"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1159\/000350292"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01873"}],"container-title":["Clinical Otolaryngology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcoa.13312","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/coa.13312","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/coa.13312","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/coa.13312","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T21:30:34Z","timestamp":1694122234000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/coa.13312"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,18]]},"references-count":30,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,5]]}},"alternative-id":["10.1111\/coa.13312"],"URL":"https:\/\/doi.org\/10.1111\/coa.13312","archive":["Portico"],"relation":{},"ISSN":["1749-4478","1749-4486"],"issn-type":[{"value":"1749-4478","type":"print"},{"value":"1749-4486","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,18]]},"assertion":[{"value":"2018-08-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}