{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:53:05Z","timestamp":1747183985202,"version":"3.40.5"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,5,24]],"date-time":"2019-05-24T00:00:00Z","timestamp":1558656000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Otolaryngology"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To study the inflammatory infiltrates associated with the different stages of laryngeal carcinogenesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>Observational, matched case\u2010control study of histopathologic specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>An academic referral centre.<\/jats:p><\/jats:sec><jats:sec><jats:title>Participants<\/jats:title><jats:p>A total of 45 patients who underwent removal of glottic lesions between 2008 and 2015. Patients were enrolled and categorised into three matched groups according to lesions' histopathologic diagnoses, 15 patients in each group: benign, pre\u2010malignant and squamous cell carcinoma (SCC). Matching was based on age, gender and pack\u2010years.<\/jats:p><\/jats:sec><jats:sec><jats:title>Main outcome measures<\/jats:title><jats:p>Immunohistochemistry staining using monoclonal antibodies against CD4, CD8, CD68, CD20 and S100 representing T\u2010helper cells, cytotoxic T cells, macrophages, B cells and dendritic cells, respectively. Cell counts and distributions were measured and compared between groups. Correlations between the different cells were examined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The predominant cell type was CD8+, followed by CD68+ and CD4+. All inflammatory cells increased significantly in number in SCC (<jats:italic>P<\/jats:italic>\u2010value\u00a0&lt;\u00a00.001), with no significant difference between benign and pre\u2010malignant groups. Strong correlations between the different cells were demonstrated only in the malignant group. S100+ cells correlated with both T\u2010cell subsets, CD4+ (rho\u00a0=\u00a00.769, <jats:italic>P<\/jats:italic>\u2010value\u00a0=\u00a00.001) and CD8+ (rho\u00a0=\u00a00.697, <jats:italic>P<\/jats:italic>\u2010value\u00a0=\u00a00.0004). Infiltrates exhibited more extensive distribution in SCC compared to pre\u2010malignant and benign; CD8+ and CD68+ cells were demonstrated in both intraepithelial and stromal regions in 93% of SCC lesions (<jats:italic>P<\/jats:italic>\u2010value\u00a0=\u00a00.0001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Laryngeal carcinoma demonstrates a unique pattern of inflammatory infiltrates, with significant changes in cell counts and distribution. Leucocyte infiltrates increased significantly in the transition from laryngeal pre\u2010malignant lesion to malignancy while no significant differences were seen between benign and pre\u2010malignant lesions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/coa.13352","type":"journal-article","created":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T03:10:49Z","timestamp":1556680249000},"page":"628-638","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Local inflammatory reaction to benign, pre\u2010malignant and malignant glottic lesions: A matched case\u2010control study"],"prefix":"10.1111","volume":"44","author":[{"given":"Yonatan","family":"Lahav","sequence":"first","affiliation":[{"name":"The Department of Otolaryngology, Head and Neck Surgery Kaplan Medical Center Rehovot Israel"},{"name":"Hadassah Medical School The Hebrew University Jerusalem Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maya","family":"Shats","sequence":"additional","affiliation":[{"name":"Hadassah Medical School The Hebrew University Jerusalem Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica","family":"Huszar","sequence":"additional","affiliation":[{"name":"Hadassah Medical School The Hebrew University Jerusalem Israel"},{"name":"The Department of Pathology Kaplan Medical Center Rehovot Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaara","family":"Haimovich","sequence":"additional","affiliation":[{"name":"The Department of Otolaryngology, Head and Neck Surgery Kaplan Medical Center Rehovot Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meir","family":"Warman","sequence":"additional","affiliation":[{"name":"The Department of Otolaryngology, Head and Neck Surgery Kaplan Medical Center Rehovot Israel"},{"name":"Hadassah Medical School The Hebrew University Jerusalem Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doron","family":"Halperin","sequence":"additional","affiliation":[{"name":"The Department of Otolaryngology, Head and Neck Surgery Kaplan Medical Center Rehovot Israel"},{"name":"Hadassah Medical School The Hebrew University Jerusalem Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4704-2940","authenticated-orcid":false,"given":"Hagit","family":"Shoffel\u2010Havakuk","sequence":"additional","affiliation":[{"name":"The Department of Otolaryngology, Head and Neck Surgery Rabin Medical Center Petah Tikva Israel"},{"name":"Sackler Faculty of Medicine Tel\u2010Aviv University Tel\u2010Aviv Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,5,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2008.82"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2005.10.015"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19840201)53:3<563::AID-CNCR2820530332>3.0.CO;2-F"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-4486.2010.02181.x"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)04046-0"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01322"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2015.61.1509"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2009.05.640"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/019459988609500203"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc.2017.17"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(16)30066-3"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1602252"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40136-016-0118-0"},{"volume-title":"World Health Organization Classification of Tumours: Pathology and Genetics of Head and Neck Tumours","year":"2005","author":"Barnes L","key":"e_1_2_10_15_1"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105-010-0208-0"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2008.03111.x"},{"key":"e_1_2_10_18_1","first-page":"140","volume-title":"World Health Organization Classification of Tumours. 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