{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T15:17:24Z","timestamp":1784647044374,"version":"3.55.0"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,3,12]],"date-time":"2021-03-12T00:00:00Z","timestamp":1615507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Otolaryngology"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To identify genes that are related to delayed endolymphatic hydrops (DEH) in patients by RNA\u2010Seq analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>Observational study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>Eye &amp; ENT Hospital, Fudan University (Shanghai, China).<\/jats:p><\/jats:sec><jats:sec><jats:title>Participants<\/jats:title><jats:p>We collected the entire vestibular system from four patients with DEH who underwent labyrinthectomy. Three control samples were collected from patients with acoustic neuroma or facial neuroma treated via the translabyrinthine approach. High\u2010throughput RNA\u2010Seq analysis was performed to investigate gene expression in the pathological vestibular system.<\/jats:p><\/jats:sec><jats:sec><jats:title>Main outcome measures<\/jats:title><jats:p>Our bioinformatic analysis identified 17 genes that were upregulated and eight genes that were downregulated in patients with DEH compared with the controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The altered gene expression profile suggested that DEH is closely related to neuropathy and autoimmune disease. In addition, many of the differentially regulated genes were involved in cell adhesion, suggesting a role of cell adhesion in DEH. Immunofluorescence analysis confirmed the expression of PMP2 and CLDN19 in the cytoplasm of hair cells and scattered expression of MPZ at cell junctions. The protein expression levels were higher in specimens from patients with M\u00e9ni\u00e8re's disease and DEH compared with controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The protein expression profile of vestibular organs in patients with endolymphatic hydrops exhibited a degree of similarity to that of M\u00e9ni\u00e8re's disease. Endolymphatic hydrops is characterised by autoimmune abnormalities. DEH and M\u00e9ni\u00e8re's disease are likely to be different manifestations of the same disease, with disparate clinical symptoms. RNA\u2010Seq is a useful analytical tool to characterise the vestibular pathology based on its transcriptome.<\/jats:p><\/jats:sec>","DOI":"10.1111\/coa.13748","type":"journal-article","created":{"date-parts":[[2021,3,3]],"date-time":"2021-03-03T10:00:48Z","timestamp":1614765648000},"page":"823-833","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The transcriptome characteristics of vestibular organs from delayed endolymphatic hydrops patients (Meniere\u2019s disease)"],"prefix":"10.1111","volume":"46","author":[{"given":"Bin\u2010Jun","family":"Chen","sequence":"first","affiliation":[{"name":"ENT institute and Department of Otorhinolaryngology Eye &amp; ENT Hospital Fudan University Shanghai China"},{"name":"NHC Key Laboratory of Hearing Medicine Fudan University Shanghai China"},{"name":"Shanghai Auditory Medical Center Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010wei","family":"Luo","sequence":"additional","affiliation":[{"name":"ENT institute and Department of Otorhinolaryngology Eye &amp; 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