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Biobanking and assisted reproductive technologies (ARTs) could help overcome these challenges, but further demonstration of their potential is required to improve uptake. We used genetic and economic models to examine whether supplementing hypothetical captive populations of dibblers (\n                    <jats:italic>Parantechinus apicalis<\/jats:italic>\n                    ) and numbats (\n                    <jats:italic>Myrmecobius fasciatus<\/jats:italic>\n                    ) with biobanked founder sperm through ARTs could reduce inbreeding, lower required colony sizes, and reduce program costs. We also asked practitioners of the black\u2010footed ferret (\n                    <jats:italic>Mustela nigripes<\/jats:italic>\n                    ) captive recovery program to complete a questionnaire to examine the resources and model species research pathways required to develop an optimized biobanking protocol in the black\u2010footed ferret. We used data from this questionnaire to devise similar costed research pathways for Australian marsupials. With biobanking and assisted reproduction, inbreeding was reduced on average by between 80% and 98%, colony sizes were on average 99% smaller, and program costs were 69\u2010 to 83\u2010fold lower. Integrating biobanking made long\u2010standing captive genetic retention targets possible in marsupials (90% source population heterozygosity for a minimum of 100 years) within realistic cost frameworks. Lessons from the use of biobanking technology that contributed to the recovery of the black\u2010footed ferret include the importance of adequate research funding (US$4.2 million), extensive partnerships that provide access to facilities and equipment, colony animals, appropriate research model species, and professional and technical staff required to address knowledge gaps to deliver an optimized biobanking protocol. Applied research investment of A$133 million across marsupial research pathways could deliver biobanking protocols for 15 of Australia's most at\u2010risk marsupial species and 7 model species. The technical expertise and ex situ facilities exist to emulate the success of the black\u2010footed ferret recovery program in threatened marsupials using these research pathways. All that is needed now for significant and cost\u2010effective conservation gains is greater investment by policy makers in marsupial ARTs.\n                  <\/jats:p>","DOI":"10.1111\/cobi.14010","type":"journal-article","created":{"date-parts":[[2022,9,30]],"date-time":"2022-09-30T06:15:00Z","timestamp":1664518500000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Modeling genetic benefits and financial costs of integrating biobanking into the conservation breeding of managed marsupials"],"prefix":"10.1111","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1471-1674","authenticated-orcid":false,"given":"Lachlan G.","family":"Howell","sequence":"first","affiliation":[{"name":"School of Environmental and Life Sciences University of Newcastle  Callaghan New South Wales Australia"},{"name":"Centre for Integrative Ecology, School of Life and Environmental Sciences Deakin University  Burwood Victoria Australia"},{"name":"FAUNA Research Alliance  Kahibah New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter R.","family":"Mawson","sequence":"additional","affiliation":[{"name":"Perth Zoo, Department of Biodiversity Conservation and Attractions  South Perth Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Comizzoli","sequence":"additional","affiliation":[{"name":"Smithsonian\u2019s National Zoo and Conservation Biology Institute  Washington D.C. USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3696-6395","authenticated-orcid":false,"given":"Ryan R.","family":"Witt","sequence":"additional","affiliation":[{"name":"School of Environmental and Life Sciences University of Newcastle  Callaghan New South Wales Australia"},{"name":"FAUNA Research Alliance  Kahibah New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Frankham","sequence":"additional","affiliation":[{"name":"School of Natural Sciences Macquarie University  Sydney New South Wales Australia"},{"name":"Australian Museum  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Clulow","sequence":"additional","affiliation":[{"name":"Centre for Conservation Ecology and Genomics, Institute for Applied Ecology University of Canberra  Bruce Australian Capital Territory Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justine K.","family":"O'Brien","sequence":"additional","affiliation":[{"name":"Taronga Institute of Science and Learning Taronga Conservation Society  Mosman New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Clulow","sequence":"additional","affiliation":[{"name":"School of Environmental and Life Sciences University of Newcastle  Callaghan New South Wales Australia"},{"name":"FAUNA Research Alliance  Kahibah New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Marinari","sequence":"additional","affiliation":[{"name":"Smithsonian\u2019s National Zoo and Conservation Biology Institute  Front Royal Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John C.","family":"Rodger","sequence":"additional","affiliation":[{"name":"School of Environmental and Life Sciences University of Newcastle  Callaghan New South Wales Australia"},{"name":"FAUNA Research Alliance  Kahibah New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.107.064824"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2012.01.009"},{"key":"e_1_2_6_4_1","unstructured":"Burbidge A. 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