{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:33:35Z","timestamp":1791405215626,"version":"4.3.3"},"reference-count":21,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,5,22]],"date-time":"2013-05-22T00:00:00Z","timestamp":1369180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Contact Dermatitis"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background<\/jats:bold> Nickel was recently identified as a potent activator of dendritic cells through ligating with human Toll\u2010like receptor (TLR)\u20104.<\/jats:p><jats:p><jats:bold>Objectives<\/jats:bold> Here, we studied an extended panel of transition metals neighbouring nickel in the periodic table of elements, for their capacity to activate human monocyte\u2010derived dendritic cells (MoDCs).<\/jats:p><jats:p><jats:bold>Methods<\/jats:bold> The panel included chromium, cobalt, and palladium, all of which are known to be frequent clinical sensitizers. MoDC activation was monitored by assessment of release of the pro\u2010inflammatory mediator interleukin (IL)\u20108, a major downstream result of TLR ligation.<\/jats:p><jats:p><jats:bold>Results<\/jats:bold> The data obtained in the present study show that cobalt and palladium also have potent MoDC\u2010activating capacities, whereas copper and zinc, but not iron and chromium, have low but distinct MoDC\u2010activating potential. Involvement of endotoxin contamination in MoDC activation was excluded by <jats:italic>Limulus<\/jats:italic> assays and consistent stimulation in the presence of polymyxin B. The critical role of TLR4 in nickel\u2010induced, cobalt\u2010induced and palladium\u2010induced activation was confirmed by essentially similar stimulatory patterns obtained in an HEK293 TLR4\/MD2 transfectant cell line.<\/jats:p><jats:p><jats:bold>Conclusions<\/jats:bold> Given the adjuvant role of costimulatory danger signals, the development of contact allergies to the stimulatory metals may be facilitated by signals from direct TLR4 ligation, whereas other metal sensitizers, such as chromium, may rather depend on microbial or tissue\u2010derived cofactors to induce clinical sensitization.<\/jats:p>","DOI":"10.1111\/cod.12042","type":"journal-article","created":{"date-parts":[[2013,5,22]],"date-time":"2013-05-22T11:05:46Z","timestamp":1369220746000},"page":"331-338","source":"Crossref","is-referenced-by-count":122,"title":["Transition metal sensing by Toll\u2010like receptor\u20104: next to nickel, cobalt and palladium are potent human dendritic cell stimulators"],"prefix":"10.1111","volume":"68","author":[{"given":"Dessy","family":"Rachmawati","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hetty J.","family":"Bontkes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marleen I.","family":"Verstege","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joris","family":"Muris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. Mary E.","family":"von Blomberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rik J.","family":"Scheper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid M. W.","family":"van Hoogstraten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,5,22]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2011.01878.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2009.01572.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-011-0846-8"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2009.01963.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1919"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2011.02652.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12313366"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830251216"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.72.2.321"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-12-472"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.11.7021"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0903014"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2007.01259.x"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2012.02061.x"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1039\/b604665g"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2005.06.039"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2008.11.009"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00009-11"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/embor.2012.155"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1097\/ACI.0b013e3283079ea4"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10534-006-9029-8"}],"container-title":["Contact Dermatitis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcod.12042","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cod.12042","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T08:45:44Z","timestamp":1700729144000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cod.12042"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5,22]]},"references-count":21,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2013,6]]}},"alternative-id":["10.1111\/cod.12042"],"URL":"https:\/\/doi.org\/10.1111\/cod.12042","archive":["Portico"],"relation":{},"ISSN":["0105-1873","1600-0536"],"issn-type":[{"value":"0105-1873","type":"print"},{"value":"1600-0536","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5,22]]}}}