{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T05:33:04Z","timestamp":1791955984615,"version":"4.3.4"},"reference-count":14,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,6,28]],"date-time":"2013-06-28T00:00:00Z","timestamp":1372377600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Contact Dermatitis"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The homogeneity of methacrylates in commercial patch test preparations has not yet been investigated. Inhomogeneous patch test preparations may give rise to false\u2010negative or false\u2010positive patch test results in patients suspected of having methacrylate allergy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To investigate the homogeneity of methacrylates in commercial patch test preparations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Fresh commercial patch test preparations of methyl methacrylate (<jats:styled-content style=\"fixed-case\">MMA<\/jats:styled-content>) and 2\u2010hydroxyethyl methacrylate (2\u2010<jats:styled-content style=\"fixed-case\">HEMA<\/jats:styled-content>) from three test material suppliers in Europe were analysed quantitatively by means of normal\u2010phase high\u2010performance liquid chromatography.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The initial concentration of <jats:styled-content style=\"fixed-case\">MMA<\/jats:styled-content> in all six patch test preparations was lower than stated on the label, whereas four of six patch test preparations of 2\u2010<jats:styled-content style=\"fixed-case\">HEMA<\/jats:styled-content> were in accordance with the stated concentrations. The concentration of <jats:styled-content style=\"fixed-case\">MMA<\/jats:styled-content> increased markedly from the top segment close to the tip of the syringe to the bottom segment adjacent to the piston in four syringes (3\u20136). In contrast, syringes with 2\u2010<jats:styled-content style=\"fixed-case\">HEMA<\/jats:styled-content> maintained a constant concentration throughout the test preparation, apart from two syringes (11 and 12).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Variations in concentration and heterogeneous distribution of <jats:styled-content style=\"fixed-case\">MMA<\/jats:styled-content> and 2\u2010<jats:styled-content style=\"fixed-case\">HEMA<\/jats:styled-content> in patch test preparations may be an additional cause of variation in patch test results, besides other technical details and reading.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cod.12088","type":"journal-article","created":{"date-parts":[[2013,6,28]],"date-time":"2013-06-28T04:57:25Z","timestamp":1372395445000},"page":"239-244","source":"Crossref","is-referenced-by-count":10,"title":["Investigation of the homogeneity of methacrylate allergens in commercially available patch test preparations"],"prefix":"10.1111","volume":"69","author":[{"given":"Kristian Fredl\u00f8v","family":"Mose","sequence":"first","affiliation":[{"name":"Department of Dermatology and Allergy Centre, Odense University Hospital University of Southern Denmark  DK\u20105000 Odense C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus Ejner","family":"Andersen","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy Centre, Odense University Hospital University of Southern Denmark  DK\u20105000 Odense C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars Porskj\u00e6r","family":"Christensen","sequence":"additional","affiliation":[{"name":"Institute of Chemical Engineering, Biotechnology and Environmental Technology, University of Southern Denmark  DK\u20105230 Odense M Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,6,28]]},"reference":[{"key":"e_1_2_6_2_1","volume-title":"Patch Testing. 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