{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T10:27:34Z","timestamp":1762252054185,"version":"3.37.3"},"reference-count":13,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,3,27]],"date-time":"2014-03-27T00:00:00Z","timestamp":1395878400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006286","name":"Aage Bang Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100006286","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Copenhagen County Research Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Contact Dermatitis"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>On skin contact, nickel accumulates in the stratum corneum, where it is probably bound to proteins and amino acids. One probable contributor is filaggrin, which binds nickel avidly. Filaggrin gene (<jats:styled-content style=\"fixed-case\"><jats:italic>FLG<\/jats:italic><\/jats:styled-content>) null mutations lead to a complete lack of filaggrin production from the affected allele, and have been associated with an increased risk of nickel contact sensitization in <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>erman and <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>anish adults.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To investigate whether the experimental nickel elicitation threshold level differed between heterozygous <jats:styled-content style=\"fixed-case\"><jats:italic>FLG<\/jats:italic><\/jats:styled-content> mutation and non\u2010mutation carriers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Thirteen nickel\u2010sensitized female patients, seven heterozygous mutation carriers and six non\u2010mutation carriers (genotyped for <jats:styled-content style=\"fixed-case\">R501X<\/jats:styled-content>, 2282del4, or <jats:styled-content style=\"fixed-case\">R2447X<\/jats:styled-content>), were patch tested and performed a repeated open application test (<jats:styled-content style=\"fixed-case\">ROAT<\/jats:styled-content>) with a nickel sulfate dilution series. Logistic threshold dose\u2013response analyses were used to test for differences between the two groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>No difference was found in the dose\u2013response relationship between <jats:styled-content style=\"fixed-case\"><jats:italic>FLG<\/jats:italic><\/jats:styled-content> mutation and non\u2010mutation carriers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>On the basis of this small patient study, it appears that the elicitation threshold level for nickel is independent of <jats:styled-content style=\"fixed-case\"><jats:italic>FLG<\/jats:italic><\/jats:styled-content> null mutation single\u2010allele carrier status.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cod.12228","type":"journal-article","created":{"date-parts":[[2014,3,27]],"date-time":"2014-03-27T07:05:57Z","timestamp":1395903957000},"page":"49-53","source":"Crossref","is-referenced-by-count":12,"title":["The nickel dose\u2013response relationship by filaggrin genotype (<scp><i>FLG<\/i><\/scp>)"],"prefix":"10.1111","volume":"71","author":[{"given":"Katrine","family":"Ross\u2010Hansen","sequence":"first","affiliation":[{"name":"Department of Dermato\u2010Allergology National Allergy Research Centre, Copenhagen University Hospital Gentofte Hellerup 2900 Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeanne D.","family":"Johansen","sequence":"additional","affiliation":[{"name":"Department of Dermato\u2010Allergology National Allergy Research Centre, Copenhagen University Hospital Gentofte Hellerup 2900 Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aage","family":"V\u00f8lund","sequence":"additional","affiliation":[{"name":"Department of Dermato\u2010Allergology National Allergy Research Centre, Copenhagen University Hospital Gentofte Hellerup 2900 Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Torkil","family":"Menn\u00e9","sequence":"additional","affiliation":[{"name":"Department of Dermato\u2010Allergology National Allergy Research Centre, Copenhagen University Hospital Gentofte Hellerup 2900 Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacob P.","family":"Thyssen","sequence":"additional","affiliation":[{"name":"Department of Dermato\u2010Allergology National Allergy Research Centre, Copenhagen University Hospital Gentofte Hellerup 2900 Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,3,27]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1956.tb12809.x"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1988.tb03482.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.033969"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12398620"},{"key":"e_1_2_5_6_1","article-title":"Filaggrin is a predominant member of the denaturation\u2010resistant nickel binding proteome of human epidermis","author":"Ross\u2010Hansen K","year":"2013","journal-title":"J Invest Dermatol"},{"key":"e_1_2_5_7_1","doi-asserted-by":"crossref","first-page":"303","DOI":"10.2310\/6620.2008.08022","article-title":"Nickel sensitization, hand eczema, and loss\u2010of\u2010function mutations in the filaggrin gene","volume":"19","author":"Thyssen J 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