{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T20:23:36Z","timestamp":1772223816462,"version":"3.50.1"},"reference-count":10,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,4,9]],"date-time":"2014-04-09T00:00:00Z","timestamp":1397001600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Contact Dermatitis"],"published-print":{"date-parts":[[2014,7]]},"abstract":"Summary<\/jats:title>Background<\/jats:title>Methylchloroisothiazolinone (MCI<\/jats:styled-content>) and methylisothiazolinone (MI<\/jats:styled-content>) are the active ingredients in commonly used preservative systems (e.g. K<\/jats:styled-content>athon CG<\/jats:styled-content>\u00ae<\/jats:sup>). MCI<\/jats:styled-content>\/MI<\/jats:styled-content> is present in the E<\/jats:styled-content>uropean baseline patch test series at 100\u2009ppm aq. Since 1986, 200\u2009ppm (dose 0.006\u2009mg\/cm2<\/jats:sup>) has been used in S<\/jats:styled-content>weden without causing skin irritation. Centres in S<\/jats:styled-content>pain, the U<\/jats:styled-content>nited K<\/jats:styled-content>ingdom and I<\/jats:styled-content>reland have also used 200\u2009ppm in their baseline series.<\/jats:p><\/jats:sec>Objectives<\/jats:title>To find the optimal patch test concentration for MCI<\/jats:styled-content>\/MI<\/jats:styled-content>.<\/jats:p><\/jats:sec>Materials and methods<\/jats:title>MCI<\/jats:styled-content>\/MI<\/jats:styled-content> 100\u2009ppm aq. and MCI<\/jats:styled-content>\/MI<\/jats:styled-content> 200\u2009ppm aq. were simultaneously patch tested in 3300 consecutively tested dermatitis patients at eight E<\/jats:styled-content>uropean patch test clinics and one US<\/jats:styled-content> patch test clinic. With the F<\/jats:styled-content>inn C<\/jats:styled-content>hambers\u00ae<\/jats:sup> technique (diameter 8\u2009mm), 15\u2009\u00b5l was micropipetted on to the filter paper in the chamber. The corresponding volume for V<\/jats:styled-content>an der B<\/jats:styled-content>end\u00ae<\/jats:sup> chambers was 20\u2009\u00b5l, and that for IQ C<\/jats:styled-content>hambers\u00ae<\/jats:sup> was 25\u2009\u00b5l.<\/jats:p><\/jats:sec>Results<\/jats:title>Contact allergy to MCI<\/jats:styled-content>\/MI<\/jats:styled-content> at 100 and 200\u2009ppm was found in 1.2% and 2.1% of patients, respectively (p<\/jats:italic>\u2009<\u20090.001).<\/jats:p><\/jats:sec>Conclusions<\/jats:title>MCI<\/jats:styled-content>\/MI<\/jats:styled-content> 200\u2009ppm aq. (dose 0.006\u2009mg\/cm2<\/jats:sup>) diagnoses significantly more contact allergy than the presently used concentration of 100\u2009ppm (dose 0.003\u2009mg\/cm2<\/jats:sup>), without resulting in more adverse reactions. MCI<\/jats:styled-content>\/MI<\/jats:styled-content> at 200\u2009ppm should therefore be considered for inclusion in the E<\/jats:styled-content>uropean baseline test series.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cod.12241","type":"journal-article","created":{"date-parts":[[2014,4,9]],"date-time":"2014-04-09T09:54:03Z","timestamp":1397037243000},"page":"31-34","source":"Crossref","is-referenced-by-count":15,"title":["Patch testing with methylchloroisothiazolinone\/methylisothiazolinone 200\u2009ppm aq. detects significantly more contact allergy than 100\u2009ppm. A multicentre study within the E<\/scp>uropean E<\/scp>nvironmental and C<\/scp>ontact D<\/scp>ermatitis R<\/scp>esearch G<\/scp>roup"],"prefix":"10.1111","volume":"71","author":[{"given":"Magnus","family":"Bruze","sequence":"first","affiliation":[{"name":"Department of Occupational and Environmental Dermatology Sk\u00e5ne University Hospital, Lund University SE\u2010205 02 Malm\u00f6 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marl\u00e9ne","family":"Isaksson","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Dermatology Sk\u00e5ne University Hospital, Lund University SE\u2010205 02 Malm\u00f6 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Birgitta","family":"Gruvberger","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Dermatology Sk\u00e5ne University Hospital, Lund University SE\u2010205 02 Malm\u00f6 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus E.","family":"Andersen","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy Centre Odense University Hospital Odense 5000 Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margarida","family":"Gon\u00e7alo","sequence":"additional","affiliation":[{"name":"Department of Dermatology Faculty of Medicine, University Hospital Coimbra P3000\u2010175 Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"An","family":"Goossens","sequence":"additional","affiliation":[{"name":"Contact Allergy Unit, Department of Dermatology U.Z.K.U. Leuven 3000 Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeanne D.","family":"Johansen","sequence":"additional","affiliation":[{"name":"Department of Dermato\u2010Allergology National Research Centre, Gentofte Hospital, University of Copenhagen Hellerup 2900 Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Howard I.","family":"Maibach","sequence":"additional","affiliation":[{"name":"Department of Dermatology, School of Medicine, University of California Box 0989, Surge 100 San Francisco CA 94143\u20100989 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Rustemeyer","sequence":"additional","affiliation":[{"name":"Department of Dermatology Free University Hospital Amsterdam NL\u20101081 HV The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christophe\u2010J.","family":"Le Coz","sequence":"additional","affiliation":[{"name":"Cabinet de Dermatologie & Laboratoire de Dermatochimie 67070 Strasbourg France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian R.","family":"White","sequence":"additional","affiliation":[{"name":"Department of Cutaneous Allergy St John's Institute of Dermatology London SEI 7EH UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,4,9]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"crossref","first-page":"315","DOI":"10.2340\/0001555567315320","article-title":"Contact allergy to the active ingredients of Kathon\u00ae CG in the guinea pig","volume":"67","author":"Bruze M","year":"1987","journal-title":"Acta Derm Venereol (Stockh)"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.1987.tb01421.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.1989.tb04659.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.1990.tb01592.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2009.01509.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/DER.0b013e3182811432"},{"key":"e_1_2_5_8_1","volume-title":"Manual of Contact Dermatitis","author":"Fregert S","year":"1981"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.1991.tb01797.x"},{"key":"e_1_2_5_10_1","first-page":"283","volume-title":"Exogenous Dermatoses: Environmental Dermatitis","author":"Bruze M","year":"1990"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12184"}],"container-title":["Contact Dermatitis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcod.12241","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cod.12241","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T23:54:36Z","timestamp":1696636476000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cod.12241"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4,9]]},"references-count":10,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,7]]}},"alternative-id":["10.1111\/cod.12241"],"URL":"https:\/\/doi.org\/10.1111\/cod.12241","archive":["Portico"],"relation":{},"ISSN":["0105-1873","1600-0536"],"issn-type":[{"value":"0105-1873","type":"print"},{"value":"1600-0536","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,4,9]]}}}