{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T05:33:22Z","timestamp":1791956002484,"version":"4.3.4"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,11,18]],"date-time":"2015-11-18T00:00:00Z","timestamp":1447804800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009780","name":"Region Sk\u00e5ne","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100009780","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Contact Dermatitis"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The results from a previous study indicated the presence of several possible sensitizers formed during oxidation of the potent sensitizer<jats:italic>p<\/jats:italic>\u2010phenylenediamine (<jats:styled-content style=\"fixed-case\">PPD<\/jats:styled-content>) to which<jats:styled-content style=\"fixed-case\">PPD<\/jats:styled-content>\u2010sensitized patients might react, in various patterns.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To extract and analyse a yellow spot from a thin\u2010layer chromatogram with oxidized<jats:styled-content style=\"fixed-case\">PPD<\/jats:styled-content>, to which 6 of 14 (43%)<jats:styled-content style=\"fixed-case\">PPD<\/jats:styled-content>\u2010positive patients had reacted in a previous study, in order to identify potential sensitizer(s) and to patch test this\/these substance(s) in the 14<jats:styled-content style=\"fixed-case\">PPD<\/jats:styled-content>\u2010positive patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The yellow spot was extracted from a thin\u2010layer chromatogram of oxidized<jats:styled-content style=\"fixed-case\">PPD<\/jats:styled-content>, and two substances, suspected to be allergens, were identified by analysis with gas chromatography mass spectrometry (<jats:styled-content style=\"fixed-case\">GCMS<\/jats:styled-content>). The 14<jats:styled-content style=\"fixed-case\">PPD<\/jats:styled-content>\u2010positive patients, who had been previously tested with the thin\u2010layer chromatogram of oxidized<jats:styled-content style=\"fixed-case\">PPD<\/jats:styled-content>, participated in the investigation, and were tested with dilutions of the two substances.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">GCMS<\/jats:styled-content>analysis identified 4\u2010nitroaniline and 4,4\u2032\u2010azodianiline in the yellow spot. Of the 14<jats:styled-content style=\"fixed-case\">PPD<\/jats:styled-content>\u2010positive test patients, 5 (36%) reacted to 4\u2010nitroaniline and 9 (64%) reacted to 4,4\u2032\u2010azodianiline.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results show that 4\u2010nitroaniline and 4,4\u2032\u2010azodianiline, formed during oxidation of<jats:styled-content style=\"fixed-case\">PPD<\/jats:styled-content>, are potent sensitizers.<jats:styled-content style=\"fixed-case\">PPD<\/jats:styled-content>\u2010sensitized patients react to a high extent to concentrations equimolar to<jats:styled-content style=\"fixed-case\">PPD<\/jats:styled-content>of 4\u2010nitroaniline and 4,4\u2032\u2010azodianiline.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cod.12488","type":"journal-article","created":{"date-parts":[[2015,11,18]],"date-time":"2015-11-18T14:31:45Z","timestamp":1447857105000},"page":"76-82","source":"Crossref","is-referenced-by-count":8,"title":["Two sensitizing oxidation products of<i>p<\/i>\u2010phenylenediamine patch tested in patients allergic to<i>p<\/i>\u2010phenylenediamine"],"prefix":"10.1111","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5172-8323","authenticated-orcid":false,"given":"Ewa","family":"Young","sequence":"first","affiliation":[{"name":"Department of Occupational and Environmental Dermatology Sk\u00e5ne University Hospital, Lund University 205 02 Malm\u00f6 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Zimerson","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Dermatology Sk\u00e5ne University Hospital, Lund University 205 02 Malm\u00f6 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magnus","family":"Bruze","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Dermatology Sk\u00e5ne University Hospital, Lund University 205 02 Malm\u00f6 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"Svedman","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Dermatology Sk\u00e5ne University Hospital, Lund University 205 02 Malm\u00f6 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,11,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12164"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2013.11.016"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.13412"},{"key":"e_1_2_6_5_1","unstructured":"Abdul\u2010KarimM.Survey of ingredients present in henna products\u2009\u2013\u2009an investigation of possible contact allergens present or formed. 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