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The high level of exposure to MI<\/jats:styled-content> since then has provoked an epidemic of contact allergy to MI<\/jats:styled-content>, and an increase in MI<\/jats:styled-content>\/MCI<\/jats:styled-content> allergy. There are questions concerning the MI<\/jats:styled-content>\/MCI<\/jats:styled-content> cross\u2010reaction pattern.<\/jats:p><\/jats:sec>Objectives<\/jats:title>To bring a new perspective on the MI<\/jats:styled-content>\/MCI<\/jats:styled-content> cross\u2010reactivity issue by studying their in\u2009situ<\/jats:italic> chemical behaviour in 3D<\/jats:styled-content> reconstructed human epidermis (RHE<\/jats:styled-content>).<\/jats:p><\/jats:sec>Methods<\/jats:title>MI<\/jats:styled-content> and MCI<\/jats:styled-content> were synthesized with 13<\/jats:sup>C<\/jats:styled-content> substitution at positions C<\/jats:styled-content>\u20104\/C<\/jats:styled-content>\u20105 and C<\/jats:styled-content>\u20105, respectively. Their in\u2009situ<\/jats:italic> chemical behaviours in an RHE<\/jats:styled-content> model were followed by use of the high\u2010resolution magic angle spinning nuclear magnetic resonance technique.<\/jats:p><\/jats:sec>Results<\/jats:title>MI<\/jats:styled-content> was found to react exclusively with cysteine thiol residues, whereas MCI<\/jats:styled-content> reacted with histidines and lysines. The reaction mechanisms were found to be different for MI<\/jats:styled-content> and MCI<\/jats:styled-content>, and the adducts formed had different molecular structures.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>In RHE<\/jats:styled-content>, different MI<\/jats:styled-content>\/MCI<\/jats:styled-content> reactions towards different nucleophilic amino acids were observed, making it difficult to explain cross\u2010reactivity between MI<\/jats:styled-content> and MCI<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cod.12524","type":"journal-article","created":{"date-parts":[[2016,1,19]],"date-time":"2016-01-19T10:10:52Z","timestamp":1453198252000},"page":"159-167","source":"Crossref","is-referenced-by-count":38,"title":["In\u2009situ<\/i> chemical behaviour of methylisothiazolinone (MI<\/scp>) and methylchloroisothiazolinone (MCI<\/scp>) in reconstructed human epidermis: a new approach to the cross\u2010reactivity 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