{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T08:36:01Z","timestamp":1790152561382,"version":"4.0.1"},"reference-count":13,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,3,29]],"date-time":"2016-03-29T00:00:00Z","timestamp":1459209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24591671"],"award-info":[{"award-number":["24591671"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Contact Dermatitis"],"published-print":{"date-parts":[[2016,6]]},"abstract":"Summary<\/jats:title>Background<\/jats:title>In Japan, over 2000 users of a facial soap containing Glupearl 19S<\/jats:styled-content> (GP19S<\/jats:styled-content>), a hydrolysed wheat protein (HWP<\/jats:styled-content>), developed immediate\u2010type systemic wheat allergy (HWP\u2010IWA<\/jats:styled-content>), and \u223c70% of them developed associated contact urticaria.<\/jats:p><\/jats:sec>Objectives<\/jats:title>We investigated whether HWP\u2010IWA<\/jats:styled-content> patients cross\u2010react with other HWPs<\/jats:styled-content>, and analysed HWP antigenic characteristics.<\/jats:p><\/jats:sec>Methods<\/jats:title>We used 10 types of HWP<\/jats:styled-content> that are commercially available as cosmetic ingredients, and 16 subjects with HWP\u2010IWA<\/jats:styled-content>. We performed an enzyme\u2010linked immunosorbent assay (ELISA<\/jats:styled-content>) to evaluate the reactivity to each HWP<\/jats:styled-content>, and western blotting to evaluate the characteristics of the antigens by using HWP\u2010IWA<\/jats:styled-content> patients' serum IgE<\/jats:styled-content> antibodies. We also performed prick tests with the HWPs<\/jats:styled-content>.<\/jats:p><\/jats:sec>Results<\/jats:title>The patients reacted to four other HWPs<\/jats:styled-content> in addition to GP19S<\/jats:styled-content>, according to ELISA<\/jats:styled-content>, and this was confirmed by strong reactions in the prick tests to the same four types of HWP<\/jats:styled-content>. Smears of antigens with molecular weights ranging from the high range to the low range were seen on western blotting with the four HWPs<\/jats:styled-content> that showed strong reactions in the ELISA<\/jats:styled-content> and prick tests.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>HWP\u2010IWA<\/jats:styled-content> patients cross\u2010react with other HWPs<\/jats:styled-content>. The antigens that they cross\u2010reacted to had a molecular weight distribution similar to that of GP19S<\/jats:styled-content> present in the HWPs<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cod.12551","type":"journal-article","created":{"date-parts":[[2016,3,30]],"date-time":"2016-03-30T23:50:00Z","timestamp":1459381800000},"page":"346-352","source":"Crossref","is-referenced-by-count":17,"title":["Evaluation of the cross\u2010reactivity of antigens in Glupearl 19S<\/scp> and other hydrolysed wheat proteins in cosmetics"],"prefix":"10.1111","volume":"74","author":[{"given":"Masashi","family":"Nakamura","sequence":"first","affiliation":[{"name":"Department of Dermatology Fujita Health University School of Medicine Aichi 470\u20101192 Japan"},{"name":"General Research and Development Institute Hoyu Co., Ltd Aichi 480\u20101136 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiko","family":"Yagami","sequence":"additional","affiliation":[{"name":"Department of Dermatology Fujita Health University School of Medicine Aichi 470\u20101192 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Hara","sequence":"additional","affiliation":[{"name":"General Research and Development Institute Hoyu Co., Ltd Aichi 480\u20101136 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiyo","family":"Sano\u2010Nagai","sequence":"additional","affiliation":[{"name":"Department of Dermatology Fujita Health University School of Medicine Aichi 470\u20101192 Japan"},{"name":"Department of Integrative Medical Science for Allergic Disease Fujita Health University School of Medicine Aichi 470\u20101192 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsukane","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Dermatology Fujita Health University School of Medicine Aichi 470\u20101192 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kayoko","family":"Matsunaga","sequence":"additional","affiliation":[{"name":"Department of Dermatology Fujita Health University School of Medicine Aichi 470\u20101192 Japan"},{"name":"Department of Integrative Medical Science for Allergic Disease Fujita Health University School of Medicine Aichi 470\u20101192 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,3,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.09.035"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2011.01917.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.2332\/allergolint.13-OA-0618"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2007.00977.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-1873.2006.00830.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0536.2002.460220.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1998.tb03818.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2012.02860.x"},{"key":"e_1_2_6_10_1","unstructured":"The Reports of The Special Committee for the Safety of Protein Hydrolysates in Cosmetics by the Japanese Society of Allergology. Available at:http:\/\/www.jsaweb.jp\/modules\/news_topics\/index.php?page=article&storyid=114(last accessed 26 December 2014)."},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000341671"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12018"},{"key":"e_1_2_6_13_1","first-page":"50","article-title":"Comprehensive analyses of hydrolyzed wheat protein using shotgun proteomics","volume":"131","author":"Nakamura R","year":"2013","journal-title":"Kokuritsu Iyakuhin Shokuhin Eisei Kenkyusho Hokoku"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.2332\/allergolint.13-OA-0561"}],"container-title":["Contact Dermatitis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcod.12551","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cod.12551","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cod.12551","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cod.12551","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T23:07:23Z","timestamp":1696374443000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cod.12551"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3,29]]},"references-count":13,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,6]]}},"alternative-id":["10.1111\/cod.12551"],"URL":"https:\/\/doi.org\/10.1111\/cod.12551","archive":["Portico"],"relation":{},"ISSN":["0105-1873","1600-0536"],"issn-type":[{"value":"0105-1873","type":"print"},{"value":"1600-0536","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,3,29]]}}}