{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:53:02Z","timestamp":1773103982066,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,5,3]],"date-time":"2016-05-03T00:00:00Z","timestamp":1462233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Contact Dermatitis"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Methylisothiazolinone (<jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content>), a preservative that is commonly used in personal care products, is now recognized as an important contact allergen in both cosmetic and occupational settings.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To analyse T lymphocyte responses to <jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content>, in order to provide important information regarding the relationship between the nature of such responses and skin sensitization potency.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Proliferative responses to free <jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content> and to an <jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content>\u2013human serum albumin (<jats:styled-content style=\"fixed-case\">HSA<\/jats:styled-content>) conjugate were measured according to [<jats:styled-content style=\"fixed-case\"><jats:sup>3<\/jats:sup>H<\/jats:styled-content>]thymidine incorporation (n = 56 donors; patch test scores of + in 20, ++ in 29, and +++ in 7). Peripheral blood mononuclear cells were cultured in the presence of <jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content> (0.001\u20131 \u00b5g\/ml) or <jats:styled-content style=\"fixed-case\">MI\u2013HSA<\/jats:styled-content> (0.001\u2013100 \u00b5g\/ml). Proliferating <jats:styled-content style=\"fixed-case\">CD4<\/jats:styled-content><jats:sup>+<\/jats:sup> and <jats:styled-content style=\"fixed-case\">CD8<\/jats:styled-content><jats:sup>+<\/jats:sup> T lymphocytes were identified by flow cytometry with the intracellular marker <jats:styled-content style=\"fixed-case\">Ki<\/jats:styled-content>\u201067.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>For free <jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content>, modest positive responses were recorded for 7 of 31 donors. In contrast, <jats:styled-content style=\"fixed-case\">MI\u2013HSA<\/jats:styled-content> stimulated more marked responses in 17 of 31 donors. Characterization of positive proliferative responses showed variable patterns of proliferating <jats:styled-content style=\"fixed-case\">CD4<\/jats:styled-content><jats:sup>+<\/jats:sup> and <jats:styled-content style=\"fixed-case\">CD8<\/jats:styled-content><jats:sup>+<\/jats:sup> T lymphocytes from donors with the same patch test scores and similar maximal values.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">MI\u2013HSA<\/jats:styled-content> is able to induce secondary responses in lymphocytes drawn from sensitized subjects, and provides a more effective source of antigen than free <jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content>. Furthermore, individual donors show differential activity profiles with respect to T lymphocyte subsets.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cod.12600","type":"journal-article","created":{"date-parts":[[2016,5,4]],"date-time":"2016-05-04T16:17:18Z","timestamp":1462378638000},"page":"1-13","source":"Crossref","is-referenced-by-count":11,"title":["T lymphocyte dynamics in methylisothiazolinone\u2010allergic patients"],"prefix":"10.1111","volume":"75","author":[{"given":"Amy","family":"Popple","sequence":"first","affiliation":[{"name":"Faculty of Life Sciences University of Manchester  M13 9PT Manchester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason","family":"Williams","sequence":"additional","affiliation":[{"name":"Contact Dermatitis Investigation Unit Salford Royal NHS Foundation Trust  Salford M6 8HD Salford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gavin","family":"Maxwell","sequence":"additional","affiliation":[{"name":"Unilever Safety and Environmental Assurance Centre  Sharnbrook, Bedford MK44 1LQ Sharnbrook UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nichola","family":"Gellatly","sequence":"additional","affiliation":[{"name":"Unilever Safety and Environmental Assurance Centre  Sharnbrook, Bedford MK44 1LQ Sharnbrook UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca J.","family":"Dearman","sequence":"additional","affiliation":[{"name":"Faculty of Life Sciences University of Manchester  M13 9PT Manchester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian","family":"Kimber","sequence":"additional","affiliation":[{"name":"Faculty of Life Sciences University of Manchester  M13 9PT Manchester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,5,3]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-10302-9"},{"key":"e_1_2_5_3_1","volume-title":"Test Concentrations and Vehicles for 4350 Chemicals","author":"Groot A C","year":"2008"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/59.2.198"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/ajcd.2001.23926"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0536.2000.042006344.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2011.11.007"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2004.00547.x"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.3109\/1547691X.2015.1008656"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1159\/000233456"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.2310\/6620.2004.03054"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2009.01043.x"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5701071"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2009.187"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700581"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2003.05750.x"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12064"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/DER.0000000000000105"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.13751"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2010.01774.x"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20643"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12093"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.5.1935"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1998.00334.x"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.22.012703.104702"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2009.05.023"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2496"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2010.03386.x"},{"key":"e_1_2_5_29_1","first-page":"1053","article-title":"A unique phenotype of skin\u2010associated lymphocytes in humans. 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