{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T07:05:38Z","timestamp":1760425538189},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,1,25]],"date-time":"2017-01-25T00:00:00Z","timestamp":1485302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006286","name":"Kongelig Hofbuntmager Aage Bangs Fond","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006286","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Contact Dermatitis"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Contact allergy is characterized by local skin inflammation that, in some cases, can result in systemic immune activation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To investigate whether <jats:styled-content style=\"fixed-case\">IVIS SpectrumCT<\/jats:styled-content> analyses can be used to detect the immune response induced by contact allergens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mice were repeatedly exposed to vehicle or allergens on the ears. The local and systemic responses were analysed at different times with the <jats:styled-content style=\"fixed-case\">ProSense<\/jats:styled-content> 750 <jats:styled-content style=\"fixed-case\">FAST<\/jats:styled-content> probe in <jats:styled-content style=\"fixed-case\">IVIS SpectrumCT<\/jats:styled-content> measurements. In addition, changes in ear thickness, cytokine profile in the skin and immunological phenotype in the draining lymph nodes and spleen were determined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Local inflammation was detected by <jats:styled-content style=\"fixed-case\">ProSense<\/jats:styled-content> 750 <jats:styled-content style=\"fixed-case\">FAST<\/jats:styled-content> and correlated with changes in ear thickness, cytokine profile and immunological phenotype following exposure to the strong contact allergen 2,4\u2010dinitrofluorobenzene. Analysis of the systemic response with <jats:styled-content style=\"fixed-case\">ProSense<\/jats:styled-content> 750 <jats:styled-content style=\"fixed-case\">FAST<\/jats:styled-content> did not show any difference between allergen\u2010exposed and control mice, although fluorescence\u2010activated cell sorting analysis of the spleen showed increased numbers of \u03b3\u03b4 <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cells and <jats:styled-content style=\"fixed-case\">CD11b<\/jats:styled-content><jats:sup>+<\/jats:sup><jats:styled-content style=\"fixed-case\">CD11c<\/jats:styled-content><jats:sup>+<\/jats:sup><jats:styled-content style=\"fixed-case\">MHCII<\/jats:styled-content><jats:sup>+<\/jats:sup> cells in allergen\u2010treated mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">IVIS SpectrumCT<\/jats:styled-content> analyses with <jats:styled-content style=\"fixed-case\">ProSense<\/jats:styled-content> 750 <jats:styled-content style=\"fixed-case\">FAST<\/jats:styled-content> as the probe can be used to detect local immune responses induced by contact allergens.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cod.12746","type":"journal-article","created":{"date-parts":[[2017,1,25]],"date-time":"2017-01-25T09:22:39Z","timestamp":1485336159000},"page":"210-217","source":"Crossref","is-referenced-by-count":4,"title":["Detection of local inflammation induced by repeated exposure to contact allergens by use of <scp>IVIS S<\/scp>pectrum<scp>CT<\/scp> analyses"],"prefix":"10.1111","volume":"76","author":[{"given":"Morten M.","family":"Nielsen","sequence":"first","affiliation":[{"name":"Department of Immunology and Microbiology University of Copenhagen  2200 Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonas D.","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Department of Immunology and 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