{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:46:43Z","timestamp":1790520403755,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,2,7]],"date-time":"2017-02-07T00:00:00Z","timestamp":1486425600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Contact Dermatitis"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The patch test is considered to be the gold standard for diagnosing nickel (<jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>i) allergy. The lymphocyte transformation test (<jats:styled-content style=\"fixed-case\">LTT<\/jats:styled-content>) can also be used to detect <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>i sensitization. However, little is known about the correlation between patch test and <jats:styled-content style=\"fixed-case\">LTT<\/jats:styled-content> reactions to <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>i.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To establish and validate an <jats:styled-content style=\"fixed-case\">LTT<\/jats:styled-content> for <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>i sensitization by comparison with patch test reaction and history.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Fifty individuals without self\u2010reported \u2018<jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>i allergy\u2019 (controls) and 50 patients with self\u2010reported suspicion of <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>i allergy were included. A questionnaire\u2010aided history was taken, and patch tests with at least the baseline series and <jats:styled-content style=\"fixed-case\">LTT<\/jats:styled-content>s with various <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>i<jats:styled-content style=\"fixed-case\">SO<\/jats:styled-content><jats:sub>4<\/jats:sub> dilutions were performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the patch tests, 2 of 50 controls and 18 of 50 patients with self\u2010reported suspicion of <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>i allergy showed positive reactions to <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>i. In the <jats:styled-content style=\"fixed-case\">LTT<\/jats:styled-content>s, 2 of 50 controls and 26 of 50 patients with self\u2010reported suspicion of <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>i allergy showed positive reactions to <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>i<jats:styled-content style=\"fixed-case\">SO<\/jats:styled-content><jats:sub>4<\/jats:sub> 2.5 \u00d7 10<jats:sup>\u22125<\/jats:sup> <jats:sc>m<\/jats:sc>, and 2 of 50 controls and 17 of 50 patients with self\u2010reported suspicion of <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>i allergy showed positive reactions to <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>i<jats:styled-content style=\"fixed-case\">SO<\/jats:styled-content><jats:sub>4<\/jats:sub> 1 \u00d7 10<jats:sup>\u22125<\/jats:sup> <jats:sc>m<\/jats:sc>. Sixteen of the 18 history\u2010positive and patch test\u2010positive patients (i.e. 88% sensitivity) were also <jats:styled-content style=\"fixed-case\">LTT<\/jats:styled-content>\u2010positive, in contrast to only 2 positive LTT reactors within the 48 PT and history negative individuals (i.e. 96% specificity). [Correction added on 16 February 2017, after first online publication: The preceding sentence has been edited for language and this has been amended in this version.]<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Performing the <jats:styled-content style=\"fixed-case\">LTT<\/jats:styled-content> with optimized stimulating conditions might be a useful additional tool for the diagnosis of <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>i allergy if non\u2010sensitized controls are included.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cod.12751","type":"journal-article","created":{"date-parts":[[2017,2,8]],"date-time":"2017-02-08T02:36:37Z","timestamp":1486521397000},"page":"228-234","source":"Crossref","is-referenced-by-count":35,"title":["Evaluation of lymphocyte transformation tests as compared with patch tests in nickel allergy 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