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Predictive assays that require no or few animals are needed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To develop an alternative <jats:italic>in vitro<\/jats:italic> mouse bone marrow\u2010derived dendritic cell (<jats:styled-content style=\"fixed-case\">BMDC<\/jats:styled-content>) assay to determine the allergenic potential of chemicals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">BMDC<\/jats:styled-content>s were exposed to well\u2010known allergens and to non\u2010allergenic chemicals. Surface marker expression and cytokine release of <jats:styled-content style=\"fixed-case\">BMDC<\/jats:styled-content>s were analysed after treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Eleven tested chemicals showed a significant stimulation index (<jats:styled-content style=\"fixed-case\">SI<\/jats:styled-content>) of &gt;1.5 (accuracy, 75%; sensitivity, 69%). The four non\u2010allergens all showed a <jats:styled-content style=\"fixed-case\">SI<\/jats:styled-content> of &lt;1.5. Eight contact allergens tested showed a significant <jats:styled-content style=\"fixed-case\">SI<\/jats:styled-content> of &gt;1.5 (accuracy, 92%; sensitivity, 89%), whereas only two respiratory allergens showed a significant <jats:styled-content style=\"fixed-case\">SI<\/jats:styled-content> of &gt;1.5 (accuracy, 60%; sensitivity, 33%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results indicate that the <jats:styled-content style=\"fixed-case\">BMDC<\/jats:styled-content> assay could become a reliable test for assessment of the allergenic potential of chemicals. The next step should include the testing of further chemicals, with the aim of integrating this assay into the toolbox of <jats:italic>in vitro<\/jats:italic> methods for the evaluation of the allergenic potential of chemicals.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cod.12829","type":"journal-article","created":{"date-parts":[[2017,5,29]],"date-time":"2017-05-29T09:06:37Z","timestamp":1496048797000},"page":"311-322","source":"Crossref","is-referenced-by-count":17,"title":["<i>In vitro<\/i> detection of chemical allergens: an optimized assay using mouse bone marrow\u2010derived dendritic cells"],"prefix":"10.1111","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1393-1686","authenticated-orcid":false,"given":"Fabrice","family":"Battais","sequence":"first","affiliation":[{"name":"Department of Toxicology and Biometrology National Institute for Research and Safety (INRS)  54019 Vandoeuvre cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Huppert","sequence":"additional","affiliation":[{"name":"Department of Toxicology and Biometrology National Institute for Research and Safety (INRS)  54019 Vandoeuvre cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Langonn\u00e9","sequence":"additional","affiliation":[{"name":"Department of Toxicology and Biometrology National Institute for Research and Safety (INRS)  54019 Vandoeuvre cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Muller","sequence":"additional","affiliation":[{"name":"Department of Toxicology and Biometrology National Institute for Research and Safety (INRS)  54019 Vandoeuvre cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Sponne","sequence":"additional","affiliation":[{"name":"Department of Toxicology and Biometrology National Institute for Research and Safety (INRS)  54019 Vandoeuvre cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,5,29]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2015.00094"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00420-005-0078-z"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12553"},{"key":"e_1_2_5_5_1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1684\/ejd.2014.2472","article-title":"Skin sensitisation to fragrance ingredients: is there a role for household cleaning\/maintenance products?","volume":"25","author":"Basketter D A","year":"2015","journal-title":"Eur J Dermatol"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-1811(03)00097-5"},{"key":"e_1_2_5_7_1","unstructured":"CahillJ WilliamsJ D L MathesonM Cet al.Occupational contact dermatitis: a review of 18 years of data from an occupational dermatology clinic in Australia. 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