{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:40:50Z","timestamp":1773103250270,"version":"3.50.1"},"reference-count":9,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,3,26]],"date-time":"2018-03-26T00:00:00Z","timestamp":1522022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"University Hospital Basel and University of Basel"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Contact Dermatitis"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The patch test is the standard procedure for diagnosing delayed\u2010type sensitization. If a patch test is not possible, the flow cytometric lymphocyte proliferation test (LPT), which determines the number and type of cells responding to a specific antigen in\u2009vitro, might be considered as an alternative.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Our aim was to establish a flow cytometric LPT for the detection of delayed\u2010type allergic responses to cobalt, and to determine the correlation between stimulation indices (SIs) in LPT and the grade of patch test reactions. With the patch test as a diagnostic reference, we also assessed the sensitivity and specificity of the LPT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Fifty\u2010four patients patch tested with the baseline series including cobalt (CoCl<jats:sub>2<\/jats:sub>) were additionally tested with the flow cytometric LPT with CoCl<jats:sub>2<\/jats:sub>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There was a significant correlation between the results of both tests: <jats:italic>r<\/jats:italic><jats:sub>s<\/jats:sub>\u2009=\u20090.43; <jats:italic>P<\/jats:italic>\u2009=\u2009.001. The LPT with CoCl<jats:sub>2<\/jats:sub> showed a sensitivity of 52.6% and a specificity of 85.7%. Corresponding to the low sensitivity of the LPT, high likelihood ratios for a positive patch test reaction were reached only in cases of strong lymphocyte proliferation (SI\u2009\u2265\u200910).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In cases of clearly increased SIs, the flow cytometric LPT with CoCl<jats:sub>2<\/jats:sub> gives relevant diagnostic information, and represents a valuable alternative to patch testing.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cod.12990","type":"journal-article","created":{"date-parts":[[2018,3,26]],"date-time":"2018-03-26T07:55:30Z","timestamp":1522050930000},"page":"31-33","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Delayed\u2010type allergy to cobalt\u2014comparison of a flow cytometric lymphocyte proliferation test with patch testing"],"prefix":"10.1111","volume":"79","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0453-3229","authenticated-orcid":false,"given":"Iris","family":"Spoerri","sequence":"first","affiliation":[{"name":"Department of Biomedicine, Research Group of Dermatology University Hospital Basel  Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas J.","family":"Bircher","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Allergy Unit University Hospital Basel and University of Basel  Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanne","family":"Link","sequence":"additional","affiliation":[{"name":"Medical Immunology, Laboratory Medicine University Hospital Basel  Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingmar A. F. M.","family":"Heijnen","sequence":"additional","affiliation":[{"name":"Medical Immunology, Laboratory Medicine University Hospital Basel  Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathrin","family":"Scherer Hofmeier","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Allergy Unit University Hospital Basel and University of Basel  Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,3,26]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.14423"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12582"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2011.03862.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/1547691X.2015.1008656"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12553"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0906-6705.2005.00344.x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2012.02070.x"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12432"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/DER.0b013e31826e4591"}],"container-title":["Contact Dermatitis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcod.12990","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cod.12990","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T21:45:08Z","timestamp":1695505508000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cod.12990"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,26]]},"references-count":9,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,7]]}},"alternative-id":["10.1111\/cod.12990"],"URL":"https:\/\/doi.org\/10.1111\/cod.12990","archive":["Portico"],"relation":{},"ISSN":["0105-1873","1600-0536"],"issn-type":[{"value":"0105-1873","type":"print"},{"value":"1600-0536","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,26]]},"assertion":[{"value":"2017-11-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-02-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}