{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T21:46:04Z","timestamp":1790459164850,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,4,10]],"date-time":"2018-04-10T00:00:00Z","timestamp":1523318400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Contact Dermatitis"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Low molecular weight chemicals constitute one  of the major causes of occupational allergies. European legislation on chemicals recommends limiting the use of <jats:italic>in\u2009vivo<\/jats:italic> models for assessing the sensitizing potential of chemicals, and encourages the development of integrated alternative methods. An <jats:italic>in\u2009vitro<\/jats:italic> mouse model of bone marrow\u2010derived dendritic cells (BMDCs) that showed good accuracy (75%) and sensitivity (69%) has previously been developed to assess the sensitizing potential of chemicals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To assess the ability of BMDCs to activate T cells (TCs) in\u2009vitro.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>BMDCs pre\u2010exposed to the reference sensitizer ammonium hexachloroplatinate (AHCP) were co\u2010cultured with different subpopulations of TCs. TC activation was assessed by surface marker expression, proliferation, and cytokine release.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results showed significant activation of TCs co\u2010cultured with dendritic cells pre\u2010exposed to AHCP as evaluated by CD124 expression, proliferation, and cytokine secretion. Moreover, the response of TCs appeared to be Th2\u2010oriented. Naive TCs were shown to be involved in this response, and the removal of regulatory TCs did not improve the cell response.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The BMDCs used in this previously developed model appear to have the ability to activate TCs, confirming that the BMDC model represents a reliable assay for assessing the sensitizing potential of chemicals.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cod.12991","type":"journal-article","created":{"date-parts":[[2018,4,10]],"date-time":"2018-04-10T23:40:39Z","timestamp":1523403639000},"page":"67-75","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Activation of T cells by dendritic cells exposed to a reference sensitizer: Towards a promising model to assess the allergenic potential of chemicals"],"prefix":"10.1111","volume":"79","author":[{"given":"C\u00e9cile","family":"Huppert","sequence":"first","affiliation":[{"name":"Department of Toxicology and Biometrology National Institute for Research and Safety (INRS)  Vand\u0153uvre\u2010l\u00e8s\u2010Nancy cedex France"},{"name":"Facult\u00e9 de M\u00e9decine, INGRES, EA 7298 Universit\u00e9 de Lorraine  Vand\u0153uvre\u2010l\u00e8s\u2010Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Paris","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine, INGRES, EA 7298 Universit\u00e9 de Lorraine  Vand\u0153uvre\u2010l\u00e8s\u2010Nancy France"},{"name":"INSERM, Unit\u00e9 U1085\u2010IRSET  Rennes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Langonn\u00e9","sequence":"additional","affiliation":[{"name":"Department of Toxicology and Biometrology National Institute for Research and Safety (INRS)  Vand\u0153uvre\u2010l\u00e8s\u2010Nancy cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Muller","sequence":"additional","affiliation":[{"name":"Department of Toxicology and Biometrology National Institute for Research and Safety (INRS)  Vand\u0153uvre\u2010l\u00e8s\u2010Nancy cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julianne","family":"Mathiot","sequence":"additional","affiliation":[{"name":"Department of Toxicology and Biometrology National Institute for Research and Safety (INRS)  Vand\u0153uvre\u2010l\u00e8s\u2010Nancy cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hakima","family":"Abdessadeq","sequence":"additional","affiliation":[{"name":"Department of Toxicology and Biometrology National Institute for Research and Safety (INRS)  Vand\u0153uvre\u2010l\u00e8s\u2010Nancy cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Gagnaire","sequence":"additional","affiliation":[{"name":"Department of Toxicology and Biometrology National Institute for Research and Safety (INRS)  Vand\u0153uvre\u2010l\u00e8s\u2010Nancy cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrice","family":"Battais","sequence":"additional","affiliation":[{"name":"Department of Toxicology and Biometrology National Institute for Research and Safety (INRS)  Vand\u0153uvre\u2010l\u00e8s\u2010Nancy cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Sponne","sequence":"additional","affiliation":[{"name":"Department of Toxicology and Biometrology National Institute for Research and Safety (INRS)  Vand\u0153uvre\u2010l\u00e8s\u2010Nancy cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,4,10]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfq346"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2006.01288.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2015.00094"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2015.04.010"},{"key":"e_1_2_5_6_1","unstructured":"European Commission. 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