{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T22:58:30Z","timestamp":1776898710776,"version":"3.51.2"},"reference-count":22,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,4,10]],"date-time":"2019-04-10T00:00:00Z","timestamp":1554854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007475","name":"Canadian Dermatology Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007475","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["090066\/B\/09\/Z"],"award-info":[{"award-number":["090066\/B\/09\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["092530\/Z\/10\/Z"],"award-info":[{"award-number":["092530\/Z\/10\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Contact Dermatitis"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Polysensitivity is defined as three or more positive patch test reactions. The role of filaggrin gene (<jats:italic>FLG<\/jats:italic>) loss\u2010of\u2010function mutations in patients with polysensitivity has not been studied when barrier bypass and possible preceding barrier damage have been excluded.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To determine whether FLG loss of function mutations play a role in patients with multiple contact sensitivities when barrier bypass is excluded.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>One hundred and sixty\u2010nine patients with three or more, non\u2010cross\u2010reacting, positive patch test reactions were prospectively enrolled in this study. Exclusion criteria were a history of hand dermatitis, nickel and metal implants, and stasis dermatitis. Subjects were patch tested with the North American extended patch test series, and with other relevant haptens. DNA was obtained and sequenced for the following <jats:italic>FLG<\/jats:italic> loss\u2010of\u2010function mutations: R501X, 2282del4, R2447X, and S3247X.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>One hundred and sixty\u2010five patients were genotyped for the four <jats:italic>FLG<\/jats:italic> mutations. There was a significant association between R501X mutation and polysensitivity. For the other three tested mutations, there were no significant associations with polysensitivity. When all mutations were combined, there was a significant association between loss\u2010of\u2010function <jats:italic>FLG<\/jats:italic> mutations and polysensitivity in patients with a history of atopic dermatitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>When skin barrier bypass is excluded, there is a significant association between polysensitivity and <jats:italic>FLG<\/jats:italic> loss\u2010of\u2010function mutations.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cod.13268","type":"journal-article","created":{"date-parts":[[2019,3,14]],"date-time":"2019-03-14T08:37:07Z","timestamp":1552552627000},"page":"354-358","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Filaggrin gene loss\u2010of\u2010function mutations constitute a factor in patients with multiple contact allergies"],"prefix":"10.1111","volume":"80","author":[{"given":"Youssef","family":"Elhaji","sequence":"first","affiliation":[{"name":"Division of Clinical Dermatology and Cutaneous Science, Department of Medicine Dalhousie University  Halifax Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9104-2342","authenticated-orcid":false,"given":"Denis","family":"Sasseville","sequence":"additional","affiliation":[{"name":"Division of Dermatology and Experimental Medicine, Department of Medicine McGill University  Montreal Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melanie","family":"Pratt","sequence":"additional","affiliation":[{"name":"Division of Dermatology Department of Medicine, University of Ottawa  Ottawa Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1674-3215","authenticated-orcid":false,"given":"Yuka","family":"Asai","sequence":"additional","affiliation":[{"name":"Division of Dermatology, Department of Medicine Queen's University  Kingston Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kara","family":"Matheson","sequence":"additional","affiliation":[{"name":"Research Methods Unit, Department of Medicine Dalhousie University  Halifax Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5539-5757","authenticated-orcid":false,"given":"William H. I.","family":"McLean","sequence":"additional","affiliation":[{"name":"Division of Molecular Medicine, School of Life Sciences, Centre for Dermatology and Genetic Medicine University of Dundee  Dundee UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3896-9373","authenticated-orcid":false,"given":"Peter R.","family":"Hull","sequence":"additional","affiliation":[{"name":"Division of Clinical Dermatology and Cutaneous Science, Department of Medicine Dalhousie University  Halifax Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,4,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2007.01232.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0536.2003.00111.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.140.10.1241"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2010.01748.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2007.08252.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.12155"},{"key":"e_1_2_6_8_1","first-page":"472","article-title":"Deletion of the late cornified envelope genes LCE3B and LCE3C may promote chronic hand eczema with allergic contact dermatitis","volume":"21","author":"Molin S","year":"2011","journal-title":"J Investig Allergol Clin Immunol"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/DER.0000000000000097"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/DER.0000000000000225"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.01.031"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.13704"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12789"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1767"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2008.08730.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.12035"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12139"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.12083"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1159\/000441539"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12021"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12109"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2016.03.010"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12228"}],"container-title":["Contact Dermatitis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcod.13268","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cod.13268","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cod.13268","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cod.13268","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T16:38:12Z","timestamp":1694104692000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cod.13268"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,10]]},"references-count":22,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,6]]}},"alternative-id":["10.1111\/cod.13268"],"URL":"https:\/\/doi.org\/10.1111\/cod.13268","archive":["Portico"],"relation":{},"ISSN":["0105-1873","1600-0536"],"issn-type":[{"value":"0105-1873","type":"print"},{"value":"1600-0536","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,10]]},"assertion":[{"value":"2018-08-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}