{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T04:26:35Z","timestamp":1789878395990,"version":"4.0.1"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,5,20]],"date-time":"2019-05-20T00:00:00Z","timestamp":1558310400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["DFG 284100702"],"award-info":[{"award-number":["DFG 284100702"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["BL360\u20106"],"award-info":[{"award-number":["BL360\u20106"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201015\u2010CE15\u20100023\u201001"],"award-info":[{"award-number":["ANR\u201015\u2010CE15\u20100023\u201001"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Contact Dermatitis"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Positive patch test reactions to mixtures of oxidized terpenes containing allergenic hydroperoxides are frequently reported. However, human sensitization data for these hydroperoxides are not available.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To analyse and evaluate the human sensitization potential and potency of hydroperoxides in\u2009vitro by using human cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials\/Methods<\/jats:title><jats:p>Limonene\u20101\u2010hydroperoxide, limonene\u20102\u2010hydroperoxide, citronellol\u20107\u2010hydroperoxide, cumene hydroperoxide, 1\u2010(1\u2010hydroperoxy\u20101\u2010methylethyl)cyclohexene and mixtures of citronellol hydroperoxides (isomers at positions 6 and 7) and linalool hydroperoxides (isomers at positions 6 and 7) were studied. All compounds were synthesized except for cumene hydroperoxide, which was commercially available. Their potential and potency to activate dendritic cells (DCs) was evaluated by measuring the upregulation of CD86 and CD54 on THP\u20101 cells upon exposure in the cocultured activation test (COCAT) consisting of HaCaT cells (human keratinocyte cell line) and THP\u20101 monocytes (as a surrogate for DCs).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Hydroperoxides upregulated CD86 and\/or CD54 on cocultured THP\u20101 cells in a concentration\u2010dependent manner. The results are comparable with their sensitization potency ranking in predictive animal models.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>For the first time, the human sensitization potential and potency of several hydroperoxides were determined by the use of human cells and the COCAT method.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cod.13286","type":"journal-article","created":{"date-parts":[[2019,4,9]],"date-time":"2019-04-09T05:30:49Z","timestamp":1554787849000},"page":"97-103","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Sensitization potential and potency of terpene hydroperoxides in the cocultured activation test method"],"prefix":"10.1111","volume":"81","author":[{"given":"Jennifer","family":"Hennen","sequence":"first","affiliation":[{"name":"Department of Environmental Toxicology Trier University  Trier Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Silva e Sousa","sequence":"additional","affiliation":[{"name":"Department of Environmental Toxicology Trier University  Trier Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma","family":"Sahli","sequence":"additional","affiliation":[{"name":"Dermatochemistry Laboratory, Institute of Chemistry\u2014CNRS UMR 7177 University of Strasbourg  Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jutta","family":"Lichter","sequence":"additional","affiliation":[{"name":"Department of Environmental Toxicology Trier University  Trier Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Pierre","family":"Lepoittevin","sequence":"additional","affiliation":[{"name":"Dermatochemistry Laboratory, Institute of Chemistry\u2014CNRS UMR 7177 University of Strasbourg  Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Gim\u00e9nez\u2010Arnau","sequence":"additional","affiliation":[{"name":"Dermatochemistry Laboratory, Institute of Chemistry\u2014CNRS UMR 7177 University of Strasbourg  Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3625-0701","authenticated-orcid":false,"given":"Brunhilde","family":"Bl\u00f6meke","sequence":"additional","affiliation":[{"name":"Department of Environmental Toxicology Trier University  Trier Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,5,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/32588"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1112"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-08-371245"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12683"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.14151"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12714"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.15648"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/DER.0000000000000318"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.13037"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2012.02134.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12036"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.2310\/6620.2009.09038"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3109\/15569527.2014.979425"},{"key":"e_1_2_7_15_1","unstructured":"European Commission Scientific Committee on Consumer Products. Memorandum on Classification and Categorization of Skin Sensitisers and Grading of Test Reactions; 2005.https:\/\/ec.europa.eu\/health\/ph_risk\/committees\/04_sccp\/docs\/sccp_s_01.pdf. 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