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Those techniques with better human concordance were selected to evaluate the allergenic potential of 10 hair\u2010colouring products.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In contrast to the information on the label, chromatographic analyses identified PPD in all products. The main henna biomarker, lawsone, was not detected in one of the 10 products. Among the techniques evaluated by testing hair dye ingredients, the mDPRA, the IL\u201018 assay, GARD skin and the U\u2010SENS correlated better with human classification (concordances of 91.7%\u2010100%) and were superior to the animal testing (concordance of 78.5%). Thus, these assays were used to evaluate hair\u2010colouring products, which were classified as skin sensitizers by the use of different two\u2010of\u2010three approaches.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings highlight the toxicological consequences of, and risks associated with, the undisclosed use of PPD in henna\u2010based \u201cnatural\u201d \u201creal\u2010life\u201d products.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cod.13294","type":"journal-article","created":{"date-parts":[[2019,4,21]],"date-time":"2019-04-21T22:34:05Z","timestamp":1555886045000},"page":"194-209","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Evaluation of\n                    <i>in\u2009vitro<\/i>\n                    testing strategies for hazard assessment of the skin sensitization potential of \u201creal\u2010life\u201d mixtures: The case of henna\u2010based hair\u2010colouring products containing\n                    <i>p<\/i>\n                    \u2010phenylenediamine"],"prefix":"10.1111","volume":"81","author":[{"given":"Renato Ivan","family":"de \u00c1vila","sequence":"first","affiliation":[{"name":"Laboratory of Education and Research in In\u2009Vitro Toxicology\u2014Tox In, Faculty of Pharmacy Universidade Federal de Goi\u00e1s  Goi\u00e2nia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danillo F. M. C.","family":"Veloso","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutical Technology\u2014FarmaTec, Faculty of Pharmacy Universidade Federal de Goi\u00e1s  Goi\u00e2nia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel C.","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Laboratory of Education and Research in In\u2009Vitro Toxicology\u2014Tox In, Faculty of Pharmacy Universidade Federal de Goi\u00e1s  Goi\u00e2nia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thais\u00e2ngela L.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Laboratory of Education and Research in In\u2009Vitro Toxicology\u2014Tox In, Faculty of Pharmacy Universidade Federal de Goi\u00e1s  Goi\u00e2nia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim","family":"Lindberg","sequence":"additional","affiliation":[{"name":"Department of Immunotechnology, Medicon Village Lund University  Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malin","family":"Lindstedt","sequence":"additional","affiliation":[{"name":"Department of Immunotechnology, Medicon Village Lund University  Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone G.","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Institute of Tropical Pathology and Public Health Universidade Federal de Goi\u00e1s  Goi\u00e2nia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliana M.","family":"Lima","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutical Technology\u2014FarmaTec, Faculty of Pharmacy Universidade Federal de Goi\u00e1s  Goi\u00e2nia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0379-1325","authenticated-orcid":false,"given":"Marize C.","family":"Valadares","sequence":"additional","affiliation":[{"name":"Laboratory of Education and Research in In\u2009Vitro Toxicology\u2014Tox In, Faculty of Pharmacy Universidade Federal de Goi\u00e1s  Goi\u00e2nia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,6,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2015.03.009"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408444.2018.1429385"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2018.03.003"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2012.05.013"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.14167"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-5769(01)00173-4"},{"key":"e_1_2_7_8_1","volume-title":"The Adverse Outcome Pathway for Skin Sensitisation Initiated by Covalent Binding to Proteins","author":"OECD","year":"2012"},{"key":"e_1_2_7_9_1","volume-title":"Test No. 442C\u2014In Chemico skin sensitisation: Direct peptide reactivity assay (DPRA)","author":"OECD","year":"2015"},{"key":"e_1_2_7_10_1","volume-title":"Test No. 442D: in vitro Skin Sensitisation\u2014ARE\u2010Nrf2 luciferase test method","author":"OECD","year":"2018"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1787\/9789264264359-en"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2008.01427.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12196"},{"key":"e_1_2_7_14_1","unstructured":"European Union. 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