{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T05:50:53Z","timestamp":1768283453374,"version":"3.49.0"},"reference-count":44,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,11,21]],"date-time":"2019-11-21T00:00:00Z","timestamp":1574294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Contact Dermatitis"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>High\u2010resolution magic angle spinning (HRMAS) is a nuclear magnetic resonance (NMR) technique that enables the characterization of metabolic phenotypes\/metabolite profiles of cells, tissues, and organs, under both normal and pathological conditions, without resorting to time\u2010consuming extraction techniques.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To assess the impact of chemical skin sensitizers vs non\u2010sensitizers on the metabolome of three\u2010dimensional reconstructed human epidermis (RHE) by HRMAS NMR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Based on the SENS\u2010IS assay, 12 skin sensitizers and five non\u2010sensitizing chemicals were investigated and applied on EpiSkin RHE at the published maximal non\u2010irritating concentrations under the conditions of the test. The metabolome of RHE samples was then analyzed by HRMAS NMR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 32 different metabolites were identified; 20 of these were quantified for all samples. Statistical univariate analysis showed that the tissue content of most measured metabolites (with the exception of acetate and glucose) was different in the untreated, treated with non\u2010sensitizers, and treated with sensitizers samples. In RHE samples in contact with sensitizing chemicals, concentrations of 18 metabolites were significantly decreased. Alanine and tyrosine could not discriminate between sensitizer\u2010 and non\u2010sensitizer\u2010treated groups. A multivariate partial least\u2010squares\u2010discriminant analysis was performed on the two treated groups, discriminating sensitizing and non\u2010sensitizing chemicals with a very good R2Y value of 0.87 and a good Q2Y value of 0.70.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Data suggest that HRMAS NMR could be used to monitor the impact of chemicals, skin allergens vs non\u2010sensitizers, on the metabolome of three\u2010dimensional RHE.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cod.13415","type":"journal-article","created":{"date-parts":[[2019,10,16]],"date-time":"2019-10-16T21:19:31Z","timestamp":1571260771000},"page":"137-146","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Modifications induced by chemical skin allergens on the metabolome of reconstructed human epidermis: A pilot high\u2010resolution magic angle spinning nuclear magnetic resonance study"],"prefix":"10.1111","volume":"82","author":[{"given":"Fran\u00e7ois\u2010Marie","family":"Moussallieh","sequence":"first","affiliation":[{"name":"Institute of Chemistry CNRS UMR 7177 and University of Strasbourg  Strasbourg France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Moss","sequence":"additional","affiliation":[{"name":"Institute of Chemistry CNRS UMR 7177 and University of Strasbourg  Strasbourg France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karim","family":"Elbayed","sequence":"additional","affiliation":[{"name":"Laboratoire des Sciences de l'ing\u00e9nieur, de l'informatique et de l'imagerie (ICube) CNRS UMR 7357 and University of Strasbourg  Strasbourg France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillaume","family":"Lereaux","sequence":"additional","affiliation":[{"name":"L'Or\u00e9al R&amp;I  Aulnay\u2010sous\u2010Bois France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fleur","family":"Tourneix","sequence":"additional","affiliation":[{"name":"L'Or\u00e9al R&amp;I  Aulnay\u2010sous\u2010Bois France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9507-0700","authenticated-orcid":false,"given":"Jean\u2010Pierre","family":"Lepoittevin","sequence":"additional","affiliation":[{"name":"Institute of Chemistry CNRS UMR 7177 and University of Strasbourg  Strasbourg France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,11,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/B505793K"},{"key":"e_1_2_7_3_1","first-page":"3","volume-title":"Skin Immune System: Cutaneous Immunology and Clinical Immunodermatology","author":"Bos JD","year":"2005"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2009.05.011"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/tx7002239"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-011-0846-8"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2007.01220.x"},{"key":"e_1_2_7_8_1","unstructured":"European Commission. Regulation (EC) No 1907\/2006 of the European Parliament and of the Council of 18 December 2006 concerning the Registration Evaluation Authorisation and Restriction of Chemicals (REACH) establishing a European Chemicals Agency amending Directive 1999\/45\/EC and repealing Council Regulation (EEC) No 793\/93 and Commission Regulation (EC) No 1488\/94 as well as Council Directive 76\/769\/EEC and Commission Directives 91\/155\/EEC 93\/67\/EEC 93\/105\/EC and 2000\/21\/EC.Off J Eur Union. 2006; L396\/1 of 30.12.2006."},{"key":"e_1_2_7_9_1","first-page":"59","article-title":"Regulation (EC) No 1223\/2009 of the European Parliament and the Council of 30 November 2009 on cosmetic products","volume":"342","author":"European Commission","year":"2009","journal-title":"Off J Eur Union"},{"key":"e_1_2_7_10_1","unstructured":"OECD. The adverse outcome pathway for skin sensitisation initiated by covalent binding to proteins. Part 1: scientific evidence. 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