{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:54:56Z","timestamp":1747184096862,"version":"3.40.5"},"reference-count":8,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,7,6]],"date-time":"2020-07-06T00:00:00Z","timestamp":1593993600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Contact Dermatitis"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Patch testing is the standard method to diagnose contact allergy. Patches are applied for 48\u2009hours, which is inconvenient to patients in tropical weather. Therefore, we evaluated different patch test occlusion times with increased concentrations of an allergen to determine if occlusion time can be reduced without compromising patch test reactivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Patch test positive patients with parthenium dermatitis were enrolled and patch tested using five different concentrations (10%, 4%, 2%, 1%, and 0.5%) of parthenium extract. The patches were applied in triplicate. The first set was removed after 12\u2009hours, whereas the second and third sets were removed after 24 and 48\u2009hours, respectively. Readings were performed at 24, 48, and 96\u2009hours.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fifty patients with parthenium dermatitis were included. The positive patch test reaction rates were comparable in all three sets at 24\u2010 and 48\u2010hour readings irrespective of the occlusion time. All were positive, with 10%, 4%, and 2% concentrations at 96\u2010hour reading with an occlusion time of 12\u2009hours.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>An occlusion time of 12\u2009hours seems adequate to elicit positive patch test reaction at a 96\u2010hour reading if the concentration of patch test allergen can be increased, that is, from 1% to 2% in these patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cod.13619","type":"journal-article","created":{"date-parts":[[2020,5,26]],"date-time":"2020-05-26T10:13:27Z","timestamp":1590488007000},"page":"292-295","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Increase in concentration of patch test allergen reduces patch test occlusion time to 12\u2009hours without affecting patch test reactivity in patients with parthenium dermatitis"],"prefix":"10.1111","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8178-6388","authenticated-orcid":false,"given":"Kaushal K.","family":"Verma","sequence":"first","affiliation":[{"name":"Departments of Dermatology and Venereology All India Institute of Medical Sciences  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Himabindu","family":"Saginatham","sequence":"additional","affiliation":[{"name":"Departments of Dermatology and Venereology All India Institute of Medical Sciences  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gomathy","family":"Sethuraman","sequence":"additional","affiliation":[{"name":"Departments of Dermatology and Venereology All India Institute of Medical Sciences  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4604-3917","authenticated-orcid":false,"given":"Neetu","family":"Bhari","sequence":"additional","affiliation":[{"name":"Departments of Dermatology and Venereology All India Institute of Medical Sciences  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Kalaivani","sequence":"additional","affiliation":[{"name":"Departments of Biostatistics All India Institute of Medical Sciences  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,7,6]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"71","volume-title":"Manual of Contact Dermatitis","author":"Fregert S","year":"1981"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0536.2002.470207.x"},{"issue":"4","key":"e_1_2_6_4_1","first-page":"361","article-title":"Patch testing with nickel sulphate under occlusion for five hours","volume":"68","author":"Bruze M","year":"1988","journal-title":"Acta Derm Venerol"},{"issue":"2","key":"e_1_2_6_5_1","first-page":"92","article-title":"Six\u2010hour versus 48\u2010hour patch testing with varying concentrations of potassium dichromate","volume":"9","author":"Kosann MK","year":"1998","journal-title":"Am J Contact Dermatitis"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12241"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-1873.2004.0318.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.4103\/0019-5154.91832"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1159\/000078696"}],"container-title":["Contact Dermatitis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcod.13619","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cod.13619","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cod.13619","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cod.13619","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T17:18:59Z","timestamp":1693934339000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cod.13619"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,6]]},"references-count":8,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,10]]}},"alternative-id":["10.1111\/cod.13619"],"URL":"https:\/\/doi.org\/10.1111\/cod.13619","archive":["Portico"],"relation":{},"ISSN":["0105-1873","1600-0536"],"issn-type":[{"type":"print","value":"0105-1873"},{"type":"electronic","value":"1600-0536"}],"subject":[],"published":{"date-parts":[[2020,7,6]]},"assertion":[{"value":"2020-04-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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