{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T22:18:10Z","timestamp":1768256290303,"version":"3.49.0"},"reference-count":57,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,5,11]],"date-time":"2021-05-11T00:00:00Z","timestamp":1620691200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2016\/23\/N\/NZ6\/01488"],"award-info":[{"award-number":["2016\/23\/N\/NZ6\/01488"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009045","name":"Uniwersytet Jagiello\u0144ski Collegium Medicum","doi-asserted-by":"publisher","award":["K\/DSC\/003574"],"award-info":[{"award-number":["K\/DSC\/003574"]}],"id":[{"id":"10.13039\/100009045","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Contact Dermatitis"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Previous studies showed that natural killer (NK) cells mediate contact hypersensitivity (CHS) reaction. Many reports are showing that obesity promotes several inflammatory diseases. It was shown that diet\u2010induced obesity (DIO) aggravates classical T cell\u2013mediated CHS in mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To determine whether the high\u2010fat diet (HFD)\u2013induced obesity modulates antigen\u2010specific NK cell\u2013mediated response.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We evaluated the effect of DIO on NK cell\u2013mediated CHS reaction using a model of dinitrofluorobenzene (DNFB)\u2013induced CHS in <jats:italic>Rag1<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>Rag1<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> mice fed HFD for 8 but not for 4\u2009weeks developed aggravated CHS reaction determined by ear swelling measurement when compared to animals kept on normal diet (ND) prior to DNFB sensitization. The obese <jats:italic>Rag1<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> mice presented the adipose tissue inflammation. Furthermore, in vitro analysis showed that feeding with HFD significantly increases interferon \u03b3 (IFN\u2010\u03b3) and interleukin (IL)\u201012p70 and decreases adiponectin concentration in liver mononuclear cell (LMNC) culture supernatants. The flow cytometry analysis of LMNC revealed that HFD treatment prior to DNFB sensitization increases the percentage of NK1.1<jats:sup>+<\/jats:sup>IFN\u2010\u03b3<jats:sup>+<\/jats:sup> cell population and affects the development and maturation of NK1.1<jats:sup>+<\/jats:sup> cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In summary, current results suggest that the DIO significantly modulates the local and systemic inflammatory response, contributing to exacerbation of the CHS response mediated by liver NK cells.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cod.13871","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T09:59:34Z","timestamp":1619431174000},"page":"307-316","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Diet\u2010induced obesity aggravates <scp>NK<\/scp> cell\u2013mediated contact hypersensitivity reaction in <i>Rag1<\/i><sup><i>\u2212\/\u2212<\/i><\/sup> mice"],"prefix":"10.1111","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9676-1474","authenticated-orcid":false,"given":"Paulina","family":"Kowalczyk","sequence":"first","affiliation":[{"name":"Department of Medical Biology, Faculty of Health Sciences Jagiellonian University Medical College  Krakow Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Majewska\u2010Szczepanik","sequence":"additional","affiliation":[{"name":"Department of Medical Biology, Faculty of Health Sciences Jagiellonian University Medical College  Krakow Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Strz\u0119pa","sequence":"additional","affiliation":[{"name":"Department of Medical Biology, Faculty of Health Sciences Jagiellonian University Medical College  Krakow 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