{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:46:28Z","timestamp":1790520388382,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,3,25]],"date-time":"2022-03-25T00:00:00Z","timestamp":1648166400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Contact Dermatitis"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Diagnosis of contact allergy (CA) to Amerchol L\u2010101 (AL\u2010101), a marker for lanolin allergy, is problematic. Positive patch test reactions are frequently doubtful or weakly positive and difficult to associate with clinical relevance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To gain further insight on the allergic or irritant nature of skin reactions induced by AL\u2010101 patch test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We re\u2010tested in a dose\u2013response fashion, 10 subjects with AL\u2010101 CA and performed comprehensive transcriptomic analysis (gene arrays, quantitative real\u2010time polymerase chain reaction [qRT\u2010PCR]) of samples of their skin reactions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Eight of the 10 CA subjects reacted positively upon re\u2010test, whereas two did not react. Most of AL\u2010101 positive patch tests expressed an allergy signature with strong activation of gene modules associated with adaptive immunity and downregulation of cornification pathway genes. In addition, the breadth of gene modulation correlated with the magnitude of patch test reactions and the concentration of AL\u2010101 applied. However, we observed that some of the positive patch test reactions to AL\u2010101 expressed no\/few allergy biomarkers, suggesting the induction of an irritant skin inflammation in these samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study confirms that AL\u2010101 is an allergen that can cause both contact allergy and contact irritation. Our results also highlight that molecular profiling might help to strengthen clinical diagnosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cod.14077","type":"journal-article","created":{"date-parts":[[2022,2,20]],"date-time":"2022-02-20T07:14:12Z","timestamp":1645341252000},"page":"40-52","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Gene profiling reveals a contact allergy signature in most positive <scp>Amerchol<\/scp> L\u2010101 patch test reactions"],"prefix":"10.1111","volume":"87","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5401-2435","authenticated-orcid":false,"given":"Linda","family":"Ljungberg Silic","sequence":"first","affiliation":[{"name":"Department of Occupational and Environmental Dermatology Lund University, Sk\u00e5ne University Hospital  Malm\u00f6 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1257-2578","authenticated-orcid":false,"given":"Marine\u2010Alexia","family":"Lefevre","sequence":"additional","affiliation":[{"name":"CIRI, Centre International de Recherche en Infectiologie, (Team Epidermal Immunity and Allergy); Univ Lyon; Inserm, U1111 Universit\u00e9 Claude Bernard Lyon 1; CNRS, UMR5308; ENS de Lyon  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ola","family":"Bergendorff","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Dermatology Lund University, Sk\u00e5ne University Hospital  Malm\u00f6 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3390-0924","authenticated-orcid":false,"given":"Simon","family":"De Bernard","sequence":"additional","affiliation":[{"name":"Altrabio  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9943-1326","authenticated-orcid":false,"given":"Julien","family":"Nourikyan","sequence":"additional","affiliation":[{"name":"Altrabio  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8906-1445","authenticated-orcid":false,"given":"Laurent","family":"Buffat","sequence":"additional","affiliation":[{"name":"Altrabio  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2281-9052","authenticated-orcid":false,"given":"Audrey","family":"Nosbaum","sequence":"additional","affiliation":[{"name":"CIRI, Centre International de Recherche en Infectiologie, (Team Epidermal Immunity and Allergy); Univ Lyon; Inserm, U1111 Universit\u00e9 Claude Bernard Lyon 1; CNRS, UMR5308; ENS de Lyon  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2919-3227","authenticated-orcid":false,"given":"Magnus","family":"Bruze","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Dermatology Lund University, Sk\u00e5ne University Hospital  Malm\u00f6 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4204-803X","authenticated-orcid":false,"given":"Jean\u2010Fran\u00e7ois","family":"Nicolas","sequence":"additional","affiliation":[{"name":"CIRI, Centre International de Recherche en Infectiologie, (Team Epidermal Immunity and Allergy); Univ Lyon; Inserm, U1111 Universit\u00e9 Claude Bernard Lyon 1; CNRS, UMR5308; ENS de Lyon  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4797-0269","authenticated-orcid":false,"given":"Cecilia","family":"Svedman","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Dermatology Lund University, Sk\u00e5ne University Hospital  Malm\u00f6 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0181-8862","authenticated-orcid":false,"given":"Marc","family":"Vocanson","sequence":"additional","affiliation":[{"name":"CIRI, Centre International de Recherche en Infectiologie, (Team Epidermal Immunity and Allergy); 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