{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T03:47:56Z","timestamp":1785642476354,"version":"3.56.0"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,9,8]],"date-time":"2022-09-08T00:00:00Z","timestamp":1662595200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Contact Dermatitis"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Nickel\u2010induced proliferation or cytokine release by peripheral blood mononuclear cells may be used for in vitro diagnosis of nickel allergy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Aim of this study was to explore the nickel\u2010specific cytokine profile to further elucidate the pathogenesis of nickel allergic contact dermatitis (ACD) and to identify potential new biomarkers for nickel ACD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Peripheral blood mononuclear cells from patients and controls were cultured with T\u2010cell skewing cytokine cocktails and\/or nickel. Cytokine and chemokine concentrations were assessed in culture supernatants using validated multiplex assays. Specific cytokine production was related to history of nickel allergy and patch\u2010test results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Twenty\u2010one of the 33 analytes included in the analysis were associated with nickel allergy and included type1 (TNF\u2010\u03b1, IFN\u2010\u03b3, TNF\u2010\u03b2), type 2 (IL\u20103, IL\u20104, IL\u20105, IL\u201013), type 1\/2 (IL\u20102, IL\u201010), type 9 (IL\u20109), type 17\/1 (IL\u201017A[F], GM\u2010CSF, IL\u201021) and type 22 (IL\u201022) derived cytokines as well as the T\u2010cell\/antigen presentation cell derived factors Thymus and activation regulated chemokine (TARC), IL\u201027 and IP\u201010. Receiver operator characteristics (ROC) analysis showed that IL\u20105 was the strongest biomarker for nickel allergy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>A broad spectrum of 33 cytokines and chemokines is involved in the allergen\u2010specific immune response in nickel allergic patients. IL\u20105 remains, next to the lymphocyte proliferation test, the strongest biomarker for nickel allergy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cod.14199","type":"journal-article","created":{"date-parts":[[2022,8,12]],"date-time":"2022-08-12T03:14:25Z","timestamp":1660274065000},"page":"10-17","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Nickel allergy is associated with a broad spectrum cytokine response"],"prefix":"10.1111","volume":"88","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9170-5302","authenticated-orcid":false,"given":"Niels P. J.","family":"De Graaf","sequence":"first","affiliation":[{"name":"Department of Dermatology, Amsterdam UMC Vrije Universiteit Amsterdam  Amsterdam The Netherlands"},{"name":"Department of Dental Materials Science, Academic Centre for Dentistry Amsterdam University of Amsterdam and Vrije Universiteit Amsterdam  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanne","family":"Roffel","sequence":"additional","affiliation":[{"name":"Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam University of Amsterdam and Vrije Universiteit Amsterdam  Amsterdam The Netherlands"},{"name":"MOVE Research Institute  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3446-6138","authenticated-orcid":false,"given":"Susan","family":"Gibbs","sequence":"additional","affiliation":[{"name":"Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam University of Amsterdam and Vrije Universiteit Amsterdam  Amsterdam The Netherlands"},{"name":"MOVE Research Institute  Amsterdam The Netherlands"},{"name":"Department of Molecular Cell Biology and Immunology, Amsterdam UMC Vrije Universiteit Amsterdam  Amsterdam The Netherlands"},{"name":"Amsterdam Infection and Immunity  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3218-8031","authenticated-orcid":false,"given":"Cees J.","family":"Kleverlaan","sequence":"additional","affiliation":[{"name":"Department of Dental Materials Science, Academic Centre for Dentistry Amsterdam University of Amsterdam and Vrije Universiteit Amsterdam  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Lopez Gonzalez","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology Amsterdam UMC  Amsterdam The Netherlands"},{"name":"Cancer Center Amsterdam  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7580-0684","authenticated-orcid":false,"given":"Thomas","family":"Rustemeyer","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Amsterdam UMC Vrije Universiteit Amsterdam  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0626-4261","authenticated-orcid":false,"given":"Albert J.","family":"Feilzer","sequence":"additional","affiliation":[{"name":"Department of Dental Materials Science, Academic Centre for Dentistry Amsterdam University of Amsterdam and Vrije Universiteit Amsterdam  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3562-293X","authenticated-orcid":false,"given":"Hetty J.","family":"Bontkes","sequence":"additional","affiliation":[{"name":"Amsterdam Infection and Immunity  Amsterdam The 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