{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T18:30:27Z","timestamp":1790447427170,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,4,23]],"date-time":"2023-04-23T00:00:00Z","timestamp":1682208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Contact Dermatitis"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Fragrances are important contact allergens; however, investigation of their skin sensitization potency has been challenging in new approach methods (NAMs). Many fragrance chemicals are susceptible to autoxidation or can be metabolized by enzymes constitutively expressed in skin keratinocytes. Strong sensitizers can be formed in both of these processes. Further, keratinocytes can modulate the dendritic cell (DC) activation and maturation potential, a key event in the acquisition of contact allergy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To evaluate the 2D coculture model consisting of keratinocytes and DCs using different weak to moderate sensitizing fragrance chemicals. Further, to investigate fragrances and related oxidation products in the in vitro model and compare to in vivo data.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Chemicals were tested in the coculture activation test (COCAT), consisting of HaCaT keratinocytes and THP\u20101 cells. THP\u20101 cell surface expression of costimulatory and adhesion molecules (CD86 and CD54) collected after 24\u2009h incubation with the chemicals was analysed using flow cytometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Twenty\u2010four molecules were tested positive, three were negative (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200927). Four pairs were evaluated, with aldehydes showing a 6\u2010 to 13\u2010fold stronger responses compared to their corresponding alcohols.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Results provide insight into the activation of DC in their natural environment of keratinocytes. \u03b1,\u03b2\u2010Unsaturated alcohols were classified as weaker sensitizers compared to their corresponding aldehydes. In sum, testing of fragrances retrieved results in good agreement with in vivo data.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cod.14324","type":"journal-article","created":{"date-parts":[[2023,4,23]],"date-time":"2023-04-23T20:59:28Z","timestamp":1682283568000},"page":"1-15","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Common fragrance chemicals activate dendritic cells in coculture with keratinocytes"],"prefix":"10.1111","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5363-4590","authenticated-orcid":false,"given":"Niklas","family":"Peter","sequence":"first","affiliation":[{"name":"Department of Environmental Toxicology Trier University  Trier Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jutta","family":"Lichter","sequence":"additional","affiliation":[{"name":"Department of Environmental Toxicology Trier University  Trier Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9141-9215","authenticated-orcid":false,"given":"Lina","family":"Hagvall","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg  Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Udo","family":"Bock","sequence":"additional","affiliation":[{"name":"Department of Environmental Toxicology Trier University  Trier Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3625-0701","authenticated-orcid":false,"given":"Brunhilde","family":"Bl\u00f6meke","sequence":"additional","affiliation":[{"name":"Department of Environmental Toxicology Trier University  Trier Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,4,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.13704"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.14151"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12683"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2011.437"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.123947"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.aax5430"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2022.02.002"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/DER.0000000000000684"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2011.01945.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12127"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-018-2232-x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.19685"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.14573\/altex.1606171"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfr174"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.13286"},{"issue":"4","key":"e_1_2_8_17_1","first-page":"613","article-title":"The HaCaT\/THP\u20101 cocultured activation test (COCAT) for skin sensitization: a study of intra\u2010lab reproducibility and predictivity","volume":"36","author":"Eskes C","year":"2019","journal-title":"ALTEX"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2016.08.007"},{"issue":"1","key":"e_1_2_8_19_1","first-page":"37","article-title":"Extending the concept of predicting fish acute toxicity in vitro to the intestinal cell line RTgutGC","volume":"37","author":"Schug H","year":"2020","journal-title":"ALTEX"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/cosmetics6010003"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/taap.2000.9025"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.3730"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrestox.6b00148"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/tx700017v"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2008.08.014"},{"key":"e_1_2_8_26_1","unstructured":"CasatiS AschbergerK AsturiolD et al.Ability of Non\u2010Animal Methods for Skin Sensitisation to Detect Pre\u2010 and Pro\u2010Haptens: Report and Recommendations of an EURL ECVAM Expert Meeting.2016."},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1097\/DER.0000000000000304"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-1873.2004.00290.x"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.2310\/6620.2009.09038"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0536.2001.044006331.x"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/tx300465h"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1097\/DER.0000000000000003"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.1966.160"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2022.105244"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.1992.tb00129.x"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00370734"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-013-1090-9"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2018.04.022"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.13404"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12427"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12009"},{"issue":"4","key":"e_1_2_8_42_1","first-page":"652","article-title":"Predictivity of the kinetic direct peptide reactivity assay (kDPRA) for sensitizer potency assessment and GHS subclassification","volume":"37","author":"Natsch A","year":"2020","journal-title":"ALTEX"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2022.105128"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0536.2002.460504.x"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12234"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2016.07.018"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/tx900433n"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1021\/tx049831z"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12992"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12293"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2012.02079.x"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/jocd.13507"}],"container-title":["Contact Dermatitis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cod.14324","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T00:08:18Z","timestamp":1692490098000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cod.14324"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,23]]},"references-count":51,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,7]]}},"alternative-id":["10.1111\/cod.14324"],"URL":"https:\/\/doi.org\/10.1111\/cod.14324","archive":["Portico"],"relation":{},"ISSN":["0105-1873","1600-0536"],"issn-type":[{"value":"0105-1873","type":"print"},{"value":"1600-0536","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,23]]},"assertion":[{"value":"2023-03-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}