{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T19:29:57Z","timestamp":1789414197248,"version":"build-2803163510"},"reference-count":59,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T00:00:00Z","timestamp":1694476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004836","name":"Danmarks Frie Forskningsfond","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004836","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004410","name":"European Molecular Biology Organization","doi-asserted-by":"publisher","award":["SEG9343"],"award-info":[{"award-number":["SEG9343"]}],"id":[{"id":"10.13039\/100004410","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012331","name":"LEO Fondet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012331","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Contact Dermatitis"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Allergic contact dermatitis (ACD) is an inflammatory disease with a complex pathophysiology in which epidermal\u2010resident memory CD8<jats:sup>+<\/jats:sup> T (T<jats:sub>RM<\/jats:sub>) cells play a key role. The mechanisms involved in the activation of CD8<jats:sup>+<\/jats:sup> T<jats:sub>RM<\/jats:sub> cells during allergic flare\u2010up responses are not understood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The expression of CD100 and its ligand Plexin B2 on CD8<jats:sup>+<\/jats:sup> T<jats:sub>RM<\/jats:sub> cells and keratinocytes before and after allergen exposure was determined by flow cytometry and RT\u2010qPCR. The role of CD100 in the inflammatory response during the challenge phase of ACD was determined in a model of ACD in CD100 knockout and wild\u2010type mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We show that CD8<jats:sup>+<\/jats:sup> T<jats:sub>RM<\/jats:sub> cells express CD100 during homeostatic conditions and up\u2010regulate it following re\u2010exposure of allergen\u2010experienced skin to the experimental contact allergen 1\u2010fluoro\u20102,4\u2010dinitrobenzene (DNFB). Furthermore, Plexin B2 is up\u2010regulated on keratinocytes following exposure to some contact allergens. We show that loss of CD100 results in a reduced inflammatory response to DNFB with impaired production of IFN\u03b3, IL\u201017A, CXCL1, CXCL2, CXCL5, and IL\u20101\u03b2 and decreased recruitment of neutrophils to the epidermis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our study demonstrates that CD100 is expressed on CD8<jats:sup>+<\/jats:sup> T<jats:sub>RM<\/jats:sub> cells and is required for full activation of CD8<jats:sup>+<\/jats:sup> T<jats:sub>RM<\/jats:sub> cells and the flare\u2010up response of ACD.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cod.14414","type":"journal-article","created":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T05:59:28Z","timestamp":1694584768000},"page":"442-452","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>CD100<\/scp> boosts the inflammatory response in the challenge phase of allergic contact dermatitis in mice"],"prefix":"10.1111","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8841-8131","authenticated-orcid":false,"given":"Veronika","family":"Mraz","sequence":"first","affiliation":[{"name":"The LEO Foundation Skin Immunology Research Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences The University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7576-3884","authenticated-orcid":false,"given":"Anders B.","family":"Funch","sequence":"additional","affiliation":[{"name":"The LEO Foundation Skin Immunology Research Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences The University of Copenhagen  Copenhagen Denmark"},{"name":"Department of Dermatology and Allergy, National Allergy Research Center Copenhagen University Hospital Gentofte  Hellerup Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1435-4817","authenticated-orcid":false,"given":"Mia H.","family":"Jee","sequence":"additional","affiliation":[{"name":"The LEO Foundation Skin Immunology Research Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences The University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5757-9365","authenticated-orcid":false,"given":"Anne\u2010Sofie \u00d8.","family":"Gadsb\u00f8ll","sequence":"additional","affiliation":[{"name":"The LEO Foundation Skin Immunology Research Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences The University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0126-6241","authenticated-orcid":false,"given":"Julie F.","family":"Weber","sequence":"additional","affiliation":[{"name":"The LEO Foundation Skin Immunology Research Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences The University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6083-3475","authenticated-orcid":false,"given":"Kelvin","family":"Yeung","sequence":"additional","affiliation":[{"name":"The LEO Foundation Skin Immunology Research Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences The University of Copenhagen  Copenhagen Denmark"},{"name":"Department of Dermatology and Allergy, National Allergy Research Center Copenhagen University Hospital Gentofte  Hellerup Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1595-9698","authenticated-orcid":false,"given":"Rebecca K. 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