{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T02:04:01Z","timestamp":1779933841511,"version":"3.53.1"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T00:00:00Z","timestamp":1765670400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T00:00:00Z","timestamp":1765670400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Contact Dermatitis"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Allergic contact dermatitis (ACD) is a multifactorial inflammatory skin disorder. Polysensitisation, defined as hypersensitivity to \u2265\u20093 unrelated allergens, reflects a more severe clinical form. The LCE3C_LCE3B deletion, implicated in skin barrier dysfunction, has a yet unclear role in the susceptibility to ACD and polysensitisation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aims to evaluate the association between LCE3C_LCE3B deletion and susceptibility to ACD and polysensitisation in the Tunisian population.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A case\u2013control study included 94 confirmed ACD patients and 125 age\u2010 and sex\u2010matched controls without immune\u2010related diseases. Patch testing followed the European Baseline Series. LCE3C_LCE3B genotyping was performed using conventional three\u2010primer PCR after DNA extraction by the salting\u2010out method.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Polysensitisation was observed in 33% of ACD cases. Genotyping of the LCE3C_LCE3B deletion did not reveal statistically significant differences in allelic or genotypic frequencies between ACD cases and controls. Furthermore, no statistically significant association was found between the LCE3C_LCE3B del and polysensitisation. Similarly, no significant associations were observed between the LCE3C_LCE3B deletion and sensitisation to metals, including nickel, cobalt and chromium.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>No significant association was found between the LCE3C_LCE3B deletion and either ACD or polysensitisation. Larger studies are needed to clarify the genetic contribution to these conditions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cod.70058","type":"journal-article","created":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T01:27:16Z","timestamp":1765762036000},"page":"226-233","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genetic Insights Into Allergic Contact Dermatitis: Reassessing the Role of LCE3C_LCE3B Deletion"],"prefix":"10.1111","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7612-8172","authenticated-orcid":false,"given":"Zeineb","family":"Ben Lamine","sequence":"first","affiliation":[{"name":"Immunogenetics Unit, Faculty of Medicine Ibn Al Jazzar University of Sousse  Sousse Tunisia"},{"name":"Regional Blood Transfusion Center of Sousse University of Sousse  Sousse Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2868-776X","authenticated-orcid":false,"given":"Amen","family":"Moussa","sequence":"additional","affiliation":[{"name":"Occupational Medicine Department, Faculty of Medicine of Monastir University of Monastir  Sousse Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa","family":"Bouhoula","sequence":"additional","affiliation":[{"name":"Occupational Medicine Department University Hospital of Farhat Hached University of Sousse  Sousse Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Razene","family":"Gerisha","sequence":"additional","affiliation":[{"name":"Immunogenetics Unit, Faculty of Medicine Ibn Al Jazzar University of Sousse  Sousse Tunisia"},{"name":"Genetics Laboratory, Sahloul Hospital University of Sousse  Sousse Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarra","family":"Sabbagh","sequence":"additional","affiliation":[{"name":"Immunogenetics Unit, Faculty of Medicine Ibn Al Jazzar University of Sousse  Sousse Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imen","family":"Kacem","sequence":"additional","affiliation":[{"name":"Occupational Medicine Department University Hospital of Farhat Hached University of Sousse  Sousse Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asma","family":"Aloui","sequence":"additional","affiliation":[{"name":"Occupational Medicine Department University Hospital of Farhat Hached University of Sousse  Sousse Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Souheil","family":"Chatti","sequence":"additional","affiliation":[{"name":"Occupational Medicine Department University Hospital of Farhat Hached University of Sousse  Sousse Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5539-6979","authenticated-orcid":false,"given":"Aicha","family":"Brahem","sequence":"additional","affiliation":[{"name":"Occupational Medicine Department University Hospital of Farhat Hached University of Sousse  Sousse Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramzi","family":"Zemni","sequence":"additional","affiliation":[{"name":"Immunogenetics Unit, Faculty of Medicine Ibn Al Jazzar University of Sousse  Sousse Tunisia"},{"name":"Genetics Laboratory, Sahloul Hospital University of Sousse  Sousse Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Foued","family":"Ben Hadj Slama","sequence":"additional","affiliation":[{"name":"Immunogenetics Unit, Faculty of Medicine Ibn Al Jazzar University of Sousse  Sousse Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,14]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018\u2010011\u20100846\u20108"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.13119"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100536.2010.01723.x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12447"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.11604\/pamj.2024.48.40.35295"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13671\u2010021\u201000339\u20100"},{"key":"e_1_2_10_8_1","first-page":"301","article-title":"Genetic Susceptibility in Human Allergic Contact Sensitization","volume":"5","author":"Menn\u00e9 T.","year":"1986","journal-title":"Seminars in Dermatology"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398\u20109995.2007.01590.x"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-72451-5_61-2"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2018.02.004"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgg.2015.01.001"},{"key":"e_1_2_10_13_1","first-page":"472","article-title":"Deletion of the Late Cornified Envelope Genes LCE3B and LCE3C May Promote Chronic Hand Eczema With Allergic Contact Dermatitis","volume":"21","author":"Molin S.","year":"2011","journal-title":"Journal of Investigational Allergology & Clinical Immunology"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.14391"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403\u2010012\u20101279\u20104"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100536.2009.01573.x"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100536.2007.01155.x"},{"key":"e_1_2_10_18_1","volume-title":"Patch Testing and Prick Testing A Practical Guide Official Publication of the ICDRG","author":"Lachapelle J.\u2010M.","year":"2019"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100536.2007.01232.x"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.313"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-36335-2_2"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/DER.0000000000000111"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100536.2008.01400.x"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12623"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0032045"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.231489198"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022\u2010202X.2005.23699.x"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102164\u201014\u2010261"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2017.06.003"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12789"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.12126"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5701190"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.2310\/6620.2008.08022"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100536.2010.01748.x"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100536.2007.01242.x"}],"container-title":["Contact Dermatitis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cod.70058","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cod.70058","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cod.70058","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T01:06:38Z","timestamp":1779930398000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cod.70058"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,14]]},"references-count":34,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1111\/cod.70058"],"URL":"https:\/\/doi.org\/10.1111\/cod.70058","archive":["Portico"],"relation":{},"ISSN":["0105-1873","1600-0536"],"issn-type":[{"value":"0105-1873","type":"print"},{"value":"1600-0536","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,14]]},"assertion":[{"value":"2025-08-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}