{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:18:26Z","timestamp":1787062706103,"version":"build-2736575974"},"reference-count":52,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Contact Dermatitis"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Diisocyanates are potent sensitizers extensively used in polyurethane\u2010based materials, with numerous case reports linking them to allergic contact dermatitis (ACD). Recent extractables studies have detected residual diisocyanates in medical devices such as wound dressings. However, these assessments typically rely on non\u2010physiological extraction methods, limiting their relevance to actual skin exposure conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to develop and validate a physiologically relevant method for quantifying residual 4,4\u2032\u2010diphenylmethane diisocyanates (MDI), dicyclohexylmethane\u20104,4\u2010diisocyanates (DMDI), hexamethylene diisocyanates (HDI), toluene diisocyanates (2,4\u2010 and 2,6\u2010TDI) and isophorone diisocyanates (IPDI) leaching from wound dressings, and subsequently assess their risk of sensitisation induction and ACD elicitation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Diisocyanate standards were stabilised as di\u2010lysine adducts in an artificial sweat solution, simulating skin exposure. An optimised LC\u2013MS\/MS method was used for quantification. The method was validated across a wide concentration range and subsequently applied to 36 commercial wound dressings. Exposure levels were evaluated against toxicological thresholds for quantitative risk assessments.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The method proved reliable for all target diisocyanates (except IPDI) at concentrations \u2265 15\u2009ppb. Analysis of the samples revealed that 44% contained quantifiable levels of residual diisocyanates. Notably, over 50% of the HDI\u2010positive and 20% of MDI\u2010positive samples exceeded the acceptable exposure level (AEL) for sensitisation induction. Moreover, all samples exceeding the AEL fell above the acceptable non\u2010eliciting area dose for contact dermatitis (ANEAD).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>We developed and validated a physiologically relevant method to quantify diisocyanate residues leaching from wound dressings, demonstrating that HDI, in particular, poses a significant risk for sensitisation induction and can elicit ACD in individuals who are already sensitised.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cod.70061","type":"journal-article","created":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T02:46:39Z","timestamp":1764729999000},"page":"245-258","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Quantitative Risk Assessment of Diisocyanates Leaching From Wound Dressings Under Physiological 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