{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T18:22:40Z","timestamp":1776882160384,"version":"3.51.2"},"reference-count":37,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2013,11,5]],"date-time":"2013-11-05T00:00:00Z","timestamp":1383609600000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Colorectal Disease"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Routine prophylactic inguinal irradiation in anal cancer may cause significant toxicity associated with overtreatment bias. The aim of this study was to determine the risk of regional node metastases in anal carcinoma by identifying predictive molecular biomarkers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Clinicohistopathological data from 50 pretreatment anal carcinoma biopsies were collected. Immunohistochemical analyses with antibodies against Ki67, p53, epidermal growth factor receptor (<jats:styled-content style=\"fixed-case\">EGFR<\/jats:styled-content>) and<jats:styled-content style=\"fixed-case\">YKL<\/jats:styled-content>\u201040 were performed. Statistical correlations between biomarkers and clinicopathological features and outcomes were studied. Sentinel lymph node biopsy was performed in a subset of 36 patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All patients had undergone synchronous radiochemotherapy; tumour recurrence had developed in 26%, and 16% had died.<jats:styled-content style=\"fixed-case\">YKL<\/jats:styled-content>\u201040 tumour expression correlated with lymph node metastasis, whereas no inguinal node metastases were found in any of the (14%) patients presenting with a<jats:styled-content style=\"fixed-case\">YKL<\/jats:styled-content>\u201040\/<jats:styled-content style=\"fixed-case\">EGFR<\/jats:styled-content>\u2010negative tumour.<jats:styled-content style=\"fixed-case\">YKL<\/jats:styled-content>\u201040 expression and node metastasis were both significantly associated with shorter overall and disease\u2010free survival. Tumour grade significantly correlated with disease\u2010free survival only.<jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>, tumour histological type, Ki67, p53 and<jats:styled-content style=\"fixed-case\">EGFR<\/jats:styled-content>were not associated with outcome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">YKL<\/jats:styled-content>\u201040 expression in anal carcinoma is correlated with a poor outcome and can predict lymph node metastases. The combined absence of<jats:styled-content style=\"fixed-case\">YKL<\/jats:styled-content>\u201040 and<jats:styled-content style=\"fixed-case\">EGFR<\/jats:styled-content>expression in a first biopsy of anal carcinoma reliably selects a subset of patients without inguinal metastases. Such patients could be spared sentinel lymph node biopsy and\/or inguinal radiotherapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/codi.12289","type":"journal-article","created":{"date-parts":[[2013,5,21]],"date-time":"2013-05-21T06:58:32Z","timestamp":1369119512000},"page":"1382-1391","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["A novel biomarker\u2010based analysis reliably predicts nodal metastases in anal carcinoma: preliminary evidence of therapeutic impact"],"prefix":"10.1111","volume":"15","author":[{"given":"M.","family":"Mistrangelo","sequence":"first","affiliation":[{"name":"Digestive and Colorectal Surgery Centre of Minimal Invasive Surgery University of Turin Molinette Hospital Turin Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Senetta","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences University of Turin Turin 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